Phylogenetic and physiological characterization of deep-biosphere microorganisms in El'gygytgyn Crater Lake sediments

尽管近年来在深部次表层环境微生物学领域取得了一定的进展，但我们对深部生物圈微生物的多样性、功能及其对全球地球化学循环的影响仍知之甚少。因此，在计划的项目中，将调查El'gygytgyn火山口湖沉积物中深层生物圈微生物的丰度和多样性。这些沉积物和撞击岩石是2008年11月至2009年5月在国际综合开发项目“El'gygytgyn火山口湖科学钻探”项目范围内从El' gygytgyn湖回收的。特别令人感兴趣的是了解的发展，生存和潜在的代谢活动沿着的年代序列从最古老的到最年轻的湖泊沉积物。由于El'gygytgyn湖是由360万年前的陨石撞击形成的，因此应该考虑到这一影响，以了解自那时以来微生物生命的发展。为此，将应用微生物、分子生态学和地球化学方法对湖泊沉积物进行分析。这包括调查小规模的变化内的社区结构和潜在的代谢活动沿着湖泊沉积物年代序列。获得的数据将有助于加深我们对陨石撞击以来微生物群落的发展及其在时间和空间上的变化的认识。

Despite the progress that has been made in the field of microbiology in deep subsurface environments in recent years, we still know very little about the diversity and function of deep biosphere microorganisms and their impact on the global geochemical cycles. Therefore For this reason, in the planned project the abundance and diversity of deep biosphere microorganisms in the sediments of the El’gygytgyn Crater Lake will be investigated. The sediments and impact rocks were recovered from the El’gygytgyn Lake in the scope of the ICDP project “Scientific Drilling at El’gygytgyn Crater Lake” from November 2008 to May 2009. Of particular interest is the understanding of the development, survival and potential metabolic activity along the chronosequence from the oldest to the youngest lake sediments. Because the El’gygytgyn Lake was formed by a meteorite impact 3.6 million years ago, this impact should be taken into consideration to understand the development of microbial life since that time. For this purpose microbiological, molecular ecological and geochemical methods will be applied to analyze the lake sediments. This includes the investigation of small-scale variations within the community structure and their potential metabolic activity along the lake sediment chronosequence. The acquired data will help deepen our knowledge about on the development of microbial communities and variations within the communities in time and space since the meteorite impact.