Collaborative proposal: Developing a battery of methods for the study of the trafficking mechanisms and transformations of silver and gold nanomaterials in human cells

PI: Ioana Pavel Sizemore/Adam de la zerda提案编号：1438340/1434465机构：赖特州立大学/斯坦福大学标题：合作提案：在消费品和工业中广泛使用银纳米粒子（AgNPs），以及在医疗保健中作为诊断和治疗工具使用多种纳米粒子，需要了解它们的潜在毒性。然而，正如国家纳米技术倡议所观察到的，只有有限数量的技术可以扩大我们对纳米材料影响细胞行为的机制的理解。该项目的主要科学目标是通过开发一系列工具来研究人类细胞中具有环境代表性的AgNPs和生物医学相关的金纳米棒（aunr）的贩运机制和转化，从而填补这些知识空白。为了测试所提出方法的效率和实用性，将考虑的关键参数包括纳米材料的形状、表面电荷、剂量和暴露时间。要开发的方法将通过标准操作程序（sop）进行捕获和共享。此外，从建议的研究中获得的知识将用于建立新的课程单元，并将传播到更广泛的社区，例如通过与当地博物馆的互动，以及为100多名小六至小九学生开发两个小时的动手实验模块。一系列跨学科方法（ICP-OES/TFF、拉曼- sers /MatLab代码和电子显微镜/生物测定）将用于研究环境代表性银纳米粒子（AgNPs）和生物医学相关金纳米粒子（aunr）在人体细胞中的运输机制和转化。测试所提出方法的效率和实用性的关键参数包括纳米材料的形状、表面电荷、剂量和暴露时间。为了实现他们的目标，ppi提出了两个具体目标。在ai1中，pi将根据美国EPA标准，在细胞暴露之前和细胞运输期间的不同基准时间表征NPs的物理化学性质。在Aim 2中，研究人员将使用他们的工具，研究人类细胞中AgNPs和aunr的运输机制，作为纳米材料形状、表面电荷、剂量和暴露时间的函数。

PI: Ioana Pavel Sizemore/Adam de la ZerdaProposal Number: 1438340/1434465Institution: Wright State University/Stanford University Title: Collaborative proposal: Developing a battery of methods for the study of the trafficking mechanisms and transformations of silver and gold nanomaterials in human cells The extensive use of silver nanoparticles (AgNPs) in consumer products and industry and the use of many kinds of NPs in healthcare as diagnostic and therapeutic tools require an understanding of their potential toxicities. However, as observed by the National Nanotechnology Initiative, there are only a limited number of techniques that could expand our understanding of the mechanisms by which nanomaterials influence cell behavior. The main scientific goal of this project is to fill these knowledge gaps by developing a battery of tools for the study of the trafficking mechanisms and transformations of environmentally representative AgNPs and biomedically relevant gold nanorods (AuNRs) in human cells. Key parameters, which will be considered in order to test the efficiency and practicality of the proposed methods, will include nanomaterial shape, surface charge, dose, and exposure time. The methods that are to be developed will be captured and shared through Standard Operating Procedures (SOPs). In addition, the knowledge gained from the proposed research will be used in setting up new course modules and will be disseminated into the broader community, e.g., through interactions with a local museum and development of two hour hands-on laboratory modules for over 100 K-6 through K-9 students. A battery of interdisciplinary methods (ICP-OES/TFF, Raman-SERS/MatLab codes, and electron microscopy /bioassays) will be used for the study of the trafficking mechanisms and transformations of environmentally representative silver nanoparticles (AgNPs) and biomedically relevant gold nanoparticles (AuNRs) in human cells. Key parameters that will be considered to test the efficiency and practicality of the proposed methods will include nanomaterial shape, surface charge, dose, and exposure time. To accomplish their goals, The PIs propose two specific aims. In Aim1, the PIs will characterize the physico-chemical properties of NPs, according to the U.S. EPA standards, before cellular exposure and then at various benchmark times during cellular trafficking. In Aim 2, using their suite of tools, the investigators will study the trafficking mechanisms of AgNPs and AuNRs in human cells as a function of nanomaterial shape, surface charge, dose, and exposure time.