EAGER: Extending cell phone capabilities for sensitive detection in lateral flow assays

PI: Yager， PaulProposal: 1450187标题：EAGER：扩展手机在横向流动分析中敏感检测的能力意义：分散当今最复杂的生化测量有可能彻底改变许多领域，从医学到农业；在降低社会成本、通过更频繁的测量改善结果以及进行目前不可能进行的现场测量方面，存在巨大的潜力。一种有希望的去中心化方法是将新的廉价一次性化学设备与我们的智能手机结合起来，智能手机提供“免费”的测量和分析能力，并可以立即将数据传输到任何地方。在所有这些测量中，检测过程的灵敏度决定了测量是否有用。目前的侧流装置（例如，家庭验孕棒）快速、可靠、廉价且有用，但少数商业系统只能检测一小组相对浓缩的分析物。为了成功地对低丰度分析物使用抗体结合或核酸检测试验，我们需要显著提高检测灵敏度。本项目提出的检测分析物的新方法可以为横向流格式带来这种能力。目的是通过实施这些方法来最小化成本和最大化采用速度，以便它们可以在当前或下一代智能手机上进行。智力描述：这项工作的目标是确定与移动电话相关的一组特定的附加功能是否会为智能手机在生化测量方面增加重要的功能，特别是提高当前和传统的基于横向流动的检测的灵敏度。PI提出了下一代手机可以增强我们在任何地方进行有用化学检测的能力的方法，无论是用于医学、环境监测、过程监测、实验室科学还是教育。PI将利用声波探测探测线的快速加热来测量绑定的幅度。这种方法用于光声超声成像，其中横向流条检测区被快速闪光照亮。或者，我们也可以像今天那样对明亮的光束进行光栅扫描。商用条码扫描器

PI: Yager, PaulProposal: 1450187Title: EAGER: Extending cell phone capabilities for sensitive detection in lateral flow assaysSignificance:Decentralization of today's most sophisticated biochemical measurements has the potential to revolutionize many fields, from medicine to agriculture; there is great potential to reduce societal costs, improve outcomes by more frequent measurements, and make field measurements that are currently impossible. One promising decentralization approach is to couple new inexpensive disposable chemical devices with our smart phones, which provide "free" measurement and analysis capacity, and can immediately transmit data anywhere. In all such measurement, the sensitivity of the detection process determines whether the measurement is useful. Current lateral flow devices (e.g., home pregnancy tests) are rapid, robust, inexpensive, and useful, but the few commercial systems detect a small set of relatively concentrated analytes. To successfully use antibody binding or nucleic acid detection tests for lower-abundance analytes, we need a significant increase in detection sensitivity. The proposed new methods for detecting analytes in this project could bring such capacity to the lateral flow format. The aim is to minimize cost and maximize speed of adoption by implementing these methods so that they can be carried out with current or next-generation smart phones. Intellectual Description:A goal of this work is to determine whether a particular set of additional capabilities associated with cellular telephones would add significant functionality for smart phones with respect to biochemical measurement, specifically to improving the sensitivity of current and conventional lateral flow-based assays. The PI proposes ways in which next-generation phones could enhance our ability to do useful chemical detection anywhere, whether for medicine, environmental monitoring, process monitoring, laboratory science or education.The PI will utilize acoustic detection of rapid heating of the detection lines to measure the amplitude of binding. This approach is used in photoacoustic ultrasound imaging, in which the lateral flow strip detection zone is illuminated by a rapid flash of light. Alternatively, one could raster scan a bright light beam as is done in today?s commercial barcode scanners