Career: Nanoparticle-Antibody Conjugates in Medical Imaging and Environmental Sensing

非技术性：这项由凯斯西储大学材料研究部生物材料计划资助的职业奖项将开创新型抗体-纳米颗粒结合物的先河。抗体及其结合物在科学学科中得到应用；抗体与纳米颗粒的结合为具有增强检测能力的多路复用化、微型化设备提供了一种手段。将实施生物启发的纳米制造技术，用于设计基于蛋白质的诊断，用于健康和环境监测。这项工作承诺推进纳米颗粒设计规则下的科学知识，以改进疾病和污染物检测。在未来和潜在的生物医学应用方面，所提出的生物材料在肿瘤的检测和分型方面具有巨大的潜力，或者与治疗分子结合用于药物和基因输送。此外，所提出的纳米颗粒-抗体阵列可以作为传感器来检测和/或净化各种杀虫剂、类固醇和其他污染物。先进的医学成像和环境传感设备是维持健康生活环境和社会的关键。另一个重要的好处是在一个跨学科的环境中培训和教育学生，培养团队科学、连接生物工程、合成生物学、生物医学和环境传感。K-12和公众参与将通过已建立的‘The Nanoman’多媒体推广计划来促进。技术：该奖项是为了合成和研究新型免疫传感器，并教育公众和生物分子研究和工业的下一代领导者。纳米颗粒-抗体结合物将由核蛋白组件与烟草花叶病毒成分形成的完整抗体结合域产生。软物质免疫结合物的长宽比(长宽比)将受到严格控制，因此提供了一个独特的平台来研究纳米颗粒长宽比作为增强医学成像和环境传感的设计参数。所提出的纳米颗粒-抗体结合物在生物医学中具有巨大的应用潜力，包括癌症检测、肿瘤亚型划分以及药物和基因传递。此外，生物编程纳米制造技术将被扩展到在表面产生纳米颗粒-抗体偶联物的空间阵列，用于检测水源中的污染物。疾病的筛查和检测以及环境监测对于维持健康的生活环境和社会至关重要。作为外展活动的一部分，多媒体教育项目“纳米人”将作为一个平台，通过视频和游戏提高人们对STEM研究的兴趣。研究生和本科生培训的重点是合成生物学和生物分子工程，重点是协作和跨学科研究。

Non-technical:This CAREER award funded by the Biomaterials program in the Division of Materials Research to Case Western Reserve University will pioneer novel antibody-nanoparticle conjugates. Antibodies and their conjugates find applications across scientific disciplines; the combination of antibodies with nanoparticles provides a means for multiplexed, miniaturized devices with enhanced detection capabilities. A biology-inspired nanofabrication technology will be implemented for the design of protein-based diagnostics for health and environment monitoring. This work promises to advance the scientific knowledge underlying nanoparticle design rules for improved disease and pollutant detection. With respect to future and potential biomedical applications, the proposed biomaterials hold great potential for detection and sub-typing of tumors, or combined with therapeutic molecules for drug- and gene-delivery. Furthermore, the proposed nanoparticle-antibody arrays could find applications as sensors to detect and/or purify various pesticides, steroids, and other pollutants. Advanced medical imaging and environment sensing devices is the key to maintain healthy living environments and societies. An important further benefit is the training and education of students in an interdisciplinary environment fostering team sciences, bridging bioengineering, synthetic biology, biomedicine, and environmental sensing. K-12 and public engagement will be promoted through the established 'The Nanoman' multimedia outreach program.Technical:This award is to synthesize and study novel immunosensors, and to educate the public and next-generation leaders in biomolecular research and industry. Nanoparticle-antibody conjugates will be produced from nucleoprotein assemblies with integrated antibody-binding domains formed by the components of the tobacco mosaic virus. The aspect ratio (length:width) of the soft matter immunoconjugates will be tightly controlled; therefore providing a unique platform to investigate nanoparticle aspect ratio as a design parameter for enhanced medical imaging and environmental sensing. The proposed nanoparticle-antibody conjugates hold great potential for applications in biomedicine, including cancer detection, sub-typing of tumors, as well as drug- and gene-delivery. Furthermore, the biology-programmed nanofabrication technique will be extended to produce spatial arrays of nanoparticle-antibody conjugates on surfaces for sensing of pollutants in water sources. Screening and detection of diseases as well as environment monitoring are critical to maintain healthy living environments and societies. As part of the outreach activities, the multimedia educational program 'The Nanoman' will serve as a platform to peak interest in STEM research through video and gaming. Graduate and undergraduate student training will focus on synthetic biology and biomolecular engineering with emphasis on collaborative and interdisciplinary research.