UNS:Novel biomaterials with light-controlled CO release for modulation of endothelial cells

该奖项的长期目标是对新的组织工程支架进行基础研究，这种支架可以改善外周动脉疾病患者的移植物生存能力。其中超过30%的患者没有可用于移植手术的存活静脉。在该奖项中，一氧化碳(CO)从纤维支架中的受控输送代表了治疗外周动脉疾病的一种独特的组织工程方法。该奖项既支持生成血管移植物的初始步骤，也支持更好地了解细胞对支架的反应的基础科学工作。此外，这项研究将与本科生和研究生的培训相结合，包括那些来自代表性不足群体的学生。这项拟议研究的具体目标是确定在细胞培养中促进内皮细胞增殖的组织工程支架成分。提出的支架的一个变革性特征是结合了独特的光活化CO释放分子(PhotoCORM)。这种策略提供了一种纤维支架，能够控制自然产生的生物信号分子(如一氧化碳)向周围细胞的输送。具体任务包括：(A)确定支持这种光致CORM技术的电纺支架成分，(B)研究局部CO剂量大小和时间对内皮细胞功能的影响，以及(C)设计和合成新型光致CORM，以更有效地输送CO。内皮细胞既可以单独培养，也可以在共培养中培养，更能代表体内的环境。这项基础研究的应用包括外周动脉疾病的治疗。该奖项由CBET生物技术和生化工程计划获得，由材料研究部生物材料计划共同资助。

1510003 Bashur, Christopher A.The long-term objective of this award is to conduct fundamental research into new tissue engineered scaffolds that that could improve graft viability in patients with peripheral artery disease. More than 30% of these patients do not have viable veins that can be used for graft surgeries. The controlled delivery of carbonmonoxide (CO) from a fibrous scaffold in this award represents a unique tissue engineering approach for treating peripheral artery disease. This award supports both the initial steps in generating the vascular graft and the basic science work to better understand the cells response to the scaffold. In addition, this research will be integrated with training of undergraduate and graduate students, including those from underrepresented groups. The specific objective of the proposed research is to determine tissue engineered scaffold compositions that promote endothelial cell proliferation in cell culture. One transformative feature of the proposed scaffold is the incorporation of unique photoactivated CO releasing molecules (photoCORM). This strategy provides a fibrous scaffold with the ability to control delivery of a naturally occurring biological signaling molecule such as carbon monoxide to the surrounding cells. The specific tasks include: (a) determining electrospun scaffold compositions that support this photoCORM technology, (b) investigating how localized CO dose magnitude and timing impacts endothelial cell function, and (c) designing and synthesizing novel photoCORMs for more effective CO delivery. The endothelial cells will be cultured both by themselves and in co-culture, which is more representative of the environment in the body. The applications of this fundamental research include treatment for peripheral artery disease.This award by the Biotechnology and Biochemical Engineering Program of CBET is co-funded by the Biomaterials Program of the Division of Materials Research.