MRI: Acquisition of a Triple Quadrupole Liquid Chromatography Mass Spectrometer System

通过主要研究仪器（MRI）和化学研究仪器和设施（CRIF）项目的这项奖励，杜克大学将获得具有串联分析能力（三重四极杆）的高性能液相色谱仪（HP-LC）质谱仪（MS）系统。该系统将用于分析从各种来源获得的物质的组成，包括从化学反应和生物来源获得的样品。液相色谱仪将混合物分离成单独的组分。然后，质谱仪电离这些成分，并通过测量离子的质量电荷比（m/z）来确定它们的质量。这是一种广泛使用的强大的分析工具，用于确定混合物或材料的成分。学生们将接受训练，在他们的研究中使用这种先进的现代仪器，为他们以后的职业生涯做好准备。该仪器将用于研究，特别是在以下领域：(a)建立化学工具来操纵金属催化氧化应激；(b)开发过渡金属催化程序，使C-、N-和O亲核试剂在C=C键上的H-X键加成（氢化）；(c)阐明金(I)活化催化的机理；(d)开发含有三配位氧化还原活性配体的钴和锰配合物，用于定向和/或对映选择性亲电原子转移过程；(e)通过基于片段的筛选文库利用蛋白质相互作用开发长链非编码RNA的多价抑制剂；(f)设计、合成和使用小分子作为聚合物材料过程的探针；(g)使用化学工具来理解细胞和发育生物学背后的信号通路。

With this award from the Major Research Instrumentation (MRI) and Chemistry Research Instrumentation and Facilities (CRIF) programs, Duke University will acquire a high performance liquid chromatograph (HP-LC) mass spectrometer (MS) system with tandem analysis capability (triple quadrupole). The system will be used to analyze the composition of substances obtained from various sources including samples obtained from chemical reactions and biological sources. The liquid chromatograph separates a mixture into its individual components. The mass spectrometer then ionizes the components and determines their mass by measuring the mass to charge ratio (m/z) of the ions. This is a widely used powerful analytical tool to determine the composition of a mixture or material. Students will be trained to use this sophisticated modern instrument in their research, preparing them for their later careers. The instrument will be used in research especially in areas such as (a) establishing chemical tools to manipulate metal-catalyzed oxidative stress; (b) developing transition metal-catalyzed procedures for the addition of the H-X bond of C-, N-, and O nucleophiles across C=C bonds (hydrofunctionalization); (c) elucidating the mechanisms of gold(I) pi-activation catalysis; (d) developing cobalt and manganese complexes that incorporate tricoordinate redox-active ligands for use in directed and/or enantioselective electrophilic atom-transfer processes; (e) developing multivalent inhibitors of long noncoding RNA using protein interactions through a fragment-based screening library; (f) designing, synthesizing, and using small molecules as probes of material processes in polymers; (g) using chemical tools to understand the signaling pathways underlying cell and developmental biology.