PECASE: Development of a New Mass Spectrometry-Based Approach for Assaying Protein Stability and Initiation of a High School Science Teacher Outreach Program

PECASE：开发一种新的基于质谱的方法来测定蛋白质稳定性并启动高中科学教师外展计划

• 批准号: 0094224
• 负责人: Michael Fitzgerald
• 金额: $ 53万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-15 至 2006-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0094224&HistoricalAwards=false
• 关键词: PECASE; Development; New; Mass; Spectrometry

AbstractProposal: 0094224 Date: March 18, 2002PI: Michael C. Fitzgerald Institution: Duke University This PECASE award to Professor Michael Fitzgerald of Duke University is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The primary research objective is to develop a mass spectrometry based method to examine protein stability and folding. The experimental technique of hydrogen-deuterium exchange combined with matrix assisted laser desorption ionization mass spectrometry has several advantages over existing techniques for studying protein folding. The long-range goals of this research are to develop a method to study complex binding interactions between multiple proteins and DNA. The educational component of this program is both innovative and well developed. Dr. Fitzgerald presents a program focused on a summer workshop for high school science teachers. He collaborated with the school district science specialist in the development of the plan, which has also received the support of eight regional principals. This research will impact the fields of mass spectrometry and biochemistry. Compared to established methods, this method is not limited by sample purity and will have greater sensitivity. It is designed to be automated and fast. Future applications of this line of research could impact the study of both disease state and normal human health.

摘要建议：0094224日期：2002年3月18日PI：迈克尔·C·菲茨杰拉德机构：杜克大学这个授予杜克大学迈克尔·菲茨杰拉德教授的PECASE奖由化学系的分析和表面化学项目支持。主要的研究目标是开发一种基于质谱学的方法来检测蛋白质的稳定性和折叠。氢-氚交换与基质辅助激光解吸电离质谱仪相结合的实验技术在研究蛋白质折叠方面比现有技术有许多优点。这项研究的长期目标是开发一种方法来研究多种蛋白质与DNA之间的复杂结合作用。该项目的教育部分既有创新性，也有很好的发展。菲茨杰拉德博士介绍了一个项目，重点是为高中理科教师举办的暑期研讨会。他与学区科学专家合作制定了该计划，该计划也得到了8名地区校长的支持。这项研究将对质谱学和生物化学领域产生影响。与已有的方法相比，该方法不受样品纯度的限制，具有更高的灵敏度。它的设计是自动化和快速的。这一系列研究的未来应用可能会对疾病状态和正常人类健康的研究产生影响。