2015 Plant Cell Dynamics Conferenc; Madison, WI - June 16-19, 2015

摘要目前迫切需要建立合理设计改良作物品种的知识库。为了实现这一目标，我们必须了解植物细胞在生长过程中如何整合代谢、细胞骨架组织和囊泡运输，以应对环境挑战。本次会议的重点是独特的，并寻求促进使用活细胞成像，图像量化，信号处理和计算模拟的创新方法。所有参与者都将进行交流，有机会展示海报，许多早期职业科学家将有机会进行口头报告。学生和博士后报告将向所有参与者教授基本的方法和数据解释。技术摘要：越来越多的植物细胞生物学家寻求更好地理解细胞器和细胞动力学的潜在机制，他们在实现研究目标方面面临着类似的技术挑战。该小组作为一个整体寻求找到最有效的方法，利用定量成像技术，生化技术和计算工具的组合，以获得对植物细胞系统的机械理解。实现这些目标需要教师、博士后、研究生和本科生在最先进的定量细胞生物学方法方面的发展、实施和培训。中西部植物细胞生物学会议为植物细胞生物学家提供了一个机会来讨论他们的最新研究，发展合作，获得当前最先进方法的实践经验，并促进新工具的开发，这将加速细胞动力学的机制分析。本次会议的重点是独特的，并寻求促进使用活细胞成像，图像量化，信号处理和计算模拟的创新方法。今年的会议重点是高分辨率光学和电子显微镜的有用研讨会。2015年会议的主讲人是器官、细胞和细胞生长分析方面公认的专家。所有参与者都将进行交流，有机会展示海报，许多早期职业科学家将有机会进行口头报告。就更广泛的影响而言，具体目标是：1)提供一个开放和包容的口头报告论坛，允许所有实验室分享研究成果。Lead pi仅限于对每个研究领域进行简短的5分钟概述，以定义关键知识以及重要的知识差距。分配给既定PI的时间限制是为了帮助专注于博士后、学生和初级PI演示。学生和博士后报告将向所有参与者教授基本的方法和数据解释。将有充足的时间进行讨论；2)为所有参与者提供一个机会，在晚上的海报会议上展示和讨论他们的结果；3)开展以超分辨率光学显微镜和电子断层成像为主题的实践演示和研讨会。

Lay AbstractThere is a strong need to develop the knowledge base that will enable the rational design of improved crop species. To attain this goal we must learn how plant cells integrate metabolism, cytoskeletal organization, and vesicle trafficking during growth and in response to environmental challenges. The focus of this meeting is unique, and seeks to promote the use of innovative methods for live cell imaging, image quantification, signal processing, and computational simulations. All participants will network, have the opportunity to present posters, and many early career scientists will have opportunities for oral presentations. Student and post-doc presentations will be geared toward teaching all participants basic methodology as well as data interpretation.Technical AbstractThere is a growing core of plant cell biologists that seek to better understand the underlying mechanisms of organelle and cell dynamics, who share similar technical challenges to meet their research goals. The group as a whole seeks to find the most effective ways to employ combinations of quantitative imaging techniques, biochemical techniques, and computational tools to gain a mechanistic understanding of plant cellular systems. Meeting these goals will require development, implementation and training of faculty, post-doctoral fellows, graduate and undergraduate students in state-of-the-art quantitative cell biology methods. The Midwest Plant Cell Biology meeting provides the opportunity for plant cell biologists to discuss their latest research, develop collaborations, gain hands-on experience with current state-of-the-art approaches, and to promote the development of new tools that will accelerate mechanistic analyses of cell dynamics. The focus of this meeting is unique, and seeks to promote the use of innovative methods for live cell imaging, image quantification, signal processing, and computational simulations. This year's meeting features useful workshops on high-resolution light and electron microscopy. The keynote speakers for the 2015 meeting are recognized experts in the analysis of organ and cell and cell growth. All participants will network, have the opportunity to present posters, and many early career scientists will have opportunities for oral presentations. The specific objectives in terms of broader impacts are: 1) To provide an open and inclusive forum for oral presentations that allows all labs to share research results. Lead PIs are limited to a brief 5 minute overview of each research area to define key knowns as well as important knowledge gaps. This limit in the time allocated to established PIs is to help focus on postdoc, student and beginning PI presentations. Student and post-doc presentations will be geared toward teaching all participants basic methodology as well as data interpretation. Ample time will be devoted for discussion; 2) To provide an opportunity for all participants to present and discuss their results in evening poster sessions; 3) To conduct hands-on demonstrations and workshop centered on the topics of super-resolution light microscopy and imaging using electron tomography.