Collaborative Research: An Integrated Experimental and Computational Approach to Discover Biomechanical Mechanisms of Leaf Epidermal Morphogenesis

合作研究：探索叶表皮形态发生生物力学机制的综合实验和计算方法

• 批准号: 1715544
• 负责人: Daniel Szymanski
• 金额: $ 91.41万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1715544&HistoricalAwards=false
• 关键词: Collaborative; Research; Integrated; Experimental; Computational

The size and shape of leaves are important agricultural traits that strongly influence crop yield. The growth of individual cells collectively determine leaf morphology, and a major challenge in biology is to understand how cellular constituents pattern the cell wall to influence local rates and directions of cell growth. This project focuses on discovering the growth control mechanisms of the outer layer of cells that form the leaf epidermis. The epidermis serves both as a waterproof layer that protects the leaf and as a growing biomechanical shell that influences the size and shape of the leaf. This project aims to understand how cell signaling and cytoskeletal patterning is controlled among adherent cells and how local growth can scale to influence tissue or whole leaf traits. The Broader Impact activities include interdisciplinary training for all members of the team (including undergraduates and high school students). A K-12 summer camp module will also be developed for the Young Nebraska Scientist Program. The Arabidopsis leaf epidermal morphogenesis system is ideally suited for major breakthroughs. However, progress has been slow because of the lack of reliable phenotyping methods to define genetic pathways and analyze the non-intuitive biomechanical interactions that occur among the cytoskeleton, cell wall, and cell geometry. The interdisciplinary research team will create a new experimental and computational approach to discover how microtubule-dependent patterning of cellulose microfibrils drives polarized growth in the epidermis. The resulting computational models will make predictions about the how cells generate the spatial and temporal heterogeneities in the cell wall that drive interdigitated growth of these jig-saw-puzzle shaped cells. Importantly, biomechanical feedback control of the cell wall on the microtubule cytoskeleton is a general feature of plant development. The proposed research will generate data and create integrated computational models that reveal how cell wall stress patterns influence cortical microtubules and tissue morphogenesis.

叶片的大小和形状是影响作物产量的重要农艺性状。单个细胞的生长共同决定了叶的形态，生物学中的一个主要挑战是了解细胞成分如何使细胞壁形成图案，从而影响细胞生长的局部速率和方向。该项目的重点是发现形成叶表皮的外层细胞的生长控制机制。表皮既是保护叶子的防水层，又是影响叶子大小和形状的生长生物力学外壳。该项目旨在了解细胞信号和细胞骨架模式是如何在粘附细胞中控制的，以及局部生长如何影响组织或整个叶片性状。 更广泛的影响活动包括对团队所有成员（包括本科生和高中生）的跨学科培训。 还将为内布拉斯加州青年科学家计划开发一个K-12夏令营模块。拟南芥叶表皮形态发生系统是理想的重大突破。然而，由于缺乏可靠的表型分析方法来定义遗传途径和分析细胞骨架，细胞壁和细胞几何形状之间发生的非直观的生物力学相互作用，进展缓慢。跨学科研究团队将创建一种新的实验和计算方法，以发现纤维素微纤维的微管依赖性图案如何驱动表皮的极化生长。由此产生的计算模型将预测细胞如何在细胞壁中产生空间和时间的异质性，这些异质性驱动这些拼图形状的细胞的交叉生长。重要的是，微管细胞骨架上细胞壁的生物力学反馈控制是植物发育的一般特征。拟议的研究将产生数据并创建集成的计算模型，揭示细胞壁应力模式如何影响皮质微管和组织形态发生。

项目成果

Microtubule-Dependent Confinement of a Cell Signaling and Actin Polymerization Control Module Regulates Polarized Cell Growth

• DOI: 10.1016/j.cub.2018.05.076
• 发表时间: 2018-08
• 期刊: Current Biology
• 影响因子: 9.2
• 作者: M. Yanagisawa;J. Alonso;D. Szymanski

Accurate 3D Cell Segmentation using Deep Feature and CRF Refinement

使用深度特征和 CRF 细化进行准确的 3D 细胞分割

• 发表时间: 2018
• 期刊: IEEE Signal Processing Society
• 作者: Jiang, Jianxiang

Reassessing the Roles of PIN Proteins and Anticlinal Microtubules during Pavement Cell Morphogenesis

• DOI: 10.1104/pp.17.01554
• 发表时间: 2018-01-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Belteton, Samuel A.;Sawchuk, Megan G.;Szymanski, Daniel B.
• 通讯作者: Szymanski, Daniel B.

The Actin Cytoskeleton: Functional Arrays for Cytoplasmic Organization and Cell Shape Control

• DOI: 10.1104/pp.17.01519
• 发表时间: 2018-01-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Szymanski, Dan;Staiger, Christopher J.
• 通讯作者: Staiger, Christopher J.

Cellular Dynamics: Cellular Systems in the Time Domain

• DOI: 10.1104/pp.17.01777
• 发表时间: 2018-01
• 期刊: Plant Physiology
• 影响因子: 7.4
• 作者: D. Szymanski;D. Bassham;T. Munnik;W. Sakamoto