Understanding how interaction of single-walled carbon nanotubes with biological interfaces in the gastrointestinal system may alter chemical bioavailability

单壁碳纳米管是一种小型人造材料，目前被用于许多消费行业。随着使用的增加，这些材料进入水生环境的可能性也在增加。这些材料的特性使它们极有可能被水生生物（如鱼类）消耗，在那里它们可能与鱼类肠道系统相互作用。该项目的总体目标是了解单壁碳纳米管如何通过使鱼类更容易受到我们环境中已经存在并与健康问题有关的化学物质（即污染物）的影响而影响肠道环境。为了实现这一目标，PI将研究单壁碳纳米管如何改变暴露的鱼类肠道的正常结构和功能，并确定这些变化是否有助于增加它们对水生环境中已经存在的其他化学物质的敏感性。单壁纳米管本质上是亲脂性的，可能会吸收我们环境中已经存在的其他水生污染物，并通过饮食途径改变鱼类的肠道。本研究的目的是通过探讨单壁碳纳米管与胃肠道系统中脂质的相互作用，从而更好地了解单壁碳纳米管与鱼类肠道的具体相互作用，这种相互作用可能导致肠道脂质组的结构和功能变化。PI还将检查胃肠道脂质谱的这种变化是否会导致化学物质的释放，这些化学物质可能被单壁碳纳米管吸附，从而增加这些污染物的生物积累。PI将通过检查单壁碳纳米管饮食暴露期间的胃肠道脂质谱来实现这些目标，确定脂质对化学物质吸收的影响，并检查纳米脂质相互作用对化学生物积累的影响。在这个项目中进行的活动预计将在国际、国家和区域范围内产生深远的影响。纳米生物相互作用研究的结果将有助于设计下一代纳米材料，利用或避免确定的下游行为。通过这种方式，PI可以继续利用纳米材料的惊人特性而不会产生有害的后果。在地方层面上，研究小组计划在这个项目中包括来自代表性不足群体的学生，并通过使用视频增加公众对纳米材料好处的认识。科学界将通过在区域会议上讲授短期课程以及在国家会议上讲授领导会议，在全国范围内参与这个项目。最后，研究小组将通过国际社会现有的教学项目，整合有关纳米材料用于解决资源贫乏国家健康问题的信息，从而扩大教育。

Bisesi, Joseph H.# 1605119 Single-walled carbon nanotubes are small man-made materials that are currently being used in a number of consumer industries. With this increased use, there is increased likelihood that these materials will make their way into aquatic environments. The materials have properties which make them highly likely to be consumed by aquatic organisms such as fish where they may interact with the fish intestinal system. The overall objective of this project is to understand how single-walled carbon nanotubes influence the intestinal environment by making fish more susceptible to chemicals (i.e. pollutants) that are already present in our environment and are associated with health concerns. To achieve this objective the PI will examine how single-walled carbon nanotubes may change the normal structure and function of the intestines of exposed fish and determine whether these changes contribute to increasing their susceptibility to other chemicals that are already present in our aquatic environments. Single-walled nanotubes are lipophilic in nature and are likely to sorb other aquatic contaminants already present in our environment as well as alter the intestinal tract of fish through dietary exposure routes. The objective of this research is to better understand the specific interactions of single-walled carbon nanotubes with fish intestines by probing their interaction with lipids in the gastrointestinal system which may result in structural and functional changes in the intestinal lipidome. The PI will also examine whether such changes in gastrointestinal lipid profiles result in release of chemicals that may be adsorbed to single-walled carbon nanotubes, increasing bioaccumulation of these contaminants. The PI will accomplish these objectives by examining gastrointestinal lipid profiles during single-walled carbon nanotube dietary exposure, determining the effects of lipids on sorption of chemicals to these materials, and examining the effects of nano-lipid interactions on chemical bioaccumulation. Activities performed in this project are expected to have far reaching impacts on an international, national, and regional scale. Results from the nano-bio interaction studies will contribute to the design of next generation nanomaterials that take advantage of, or avoid, identified downstream behavior. In this way the PI can continue to utilize the amazing properties of nanomaterials without detrimental consequences. On a local level, the research team plans to include students from underrepresented groups in this project as well as increasing public knowledge on the benefits of nanomaterials through the use of videos. The scientific community will be involved in this project on a national scale through teaching of short courses at regional meetings as well as leading sessions at national meetings. Finally, the research team will expand education by integrating information about nanomaterial uses for combating health issues in resource poor countries through existing teaching programs in international communities.