Preclinical assessment of decellularised (antigen-reduced), gene-modified porcine heart valves (C07)

这项研究计划的目标是利用家猪作为再生心脏瓣膜植入物的来源。在临床试验之前，我们将继续对GGTA1-KO猪的肺瓣膜进行持续的评估，这些瓣膜已经脱细胞和部分脱糖，最后植入我们的原位狒狒模型中。在新的资助期，我们将为来自多只KO猪的脱细胞心脏瓣膜基质开发和验证更有效的脱糖方案。这些将使用葡聚糖谱进行表征。预制抗体将在体外进行评估，使用来自潜在患者的血液和血清。然后，最终将使用我们的狒狒植入模型在体内测试各个优化的瓣膜。

The goal of this research proposal is to use the domestic pig as a source for regenerative heart valve implants. Prior to clinical trials, we will continue with the ongoing evaluations of pulmonary heart valves derived from GGTA1-ko pigs that had been decellularised and partially deglycosylated and finally implanted in our orthotopic baboon model. In the new funding period, we will develop and validate more effective deglycosylation regimes for decellularised heart valve matrices derived from multi-ko pigs. These will be characterised using glycan profiling. Preformed antibodies will be evaluated in vitro, using blood and serum from potential patients. The respective optimised valves will then finally be tested in vivo using our baboon implantation model.