Single molecule fluorescence methods for investigating conformational dynamics and allosteric effects in chaperones (A11)

在CRC1035中，我们的目标是使用先进的荧光方法来研究生物分子的构象动力学。我们已经开发了3c FRET，这样我们就可以检测到单个生物分子中的协调运动。在接下来的四年里，我们将研究Hsp70内部的变构效应，Hsp70和Hsp90之间的客户转移(与Buchner/Sattler，A03)，剪接体的动力学(与Sattler，B03)，GroEL和Groes对活细胞中蛋白质折叠以及伴侣和核糖体之间相互作用的影响(与Hayer-Hartl/Hartl，A12)，以及基于多肽的淀粉样抑制剂和小热休克蛋白的动态(与Kapurniotu，B06；Reif，B07)和HBX(与Schütz，B15)。

Within the CRC1035, our objective is to use advanced fluorescence methods to investigate the conformational dynamics of biomolecules. We have developed 3c FRET such that we can detect coordinated motions in individual biomolecules. During the next four years, we will investigate allosteric effects within Hsp70s, the transfer of clients between Hsp70 and Hsp90 (with Buchner/Sattler, A03), the dynamics of the spliceosome (with Sattler, B03), the influence of GroEL and GroES on protein folding as well as interactions between chaperones and ribosomes in living cells (with Hayer-Hartl/Hartl, A12), and the dynamics of peptide-based amyloid inhibitors and small heat shock proteins (with Kapurniotu, B06; Reif, B07) and HBx (with Schütz, B15).