New Tools for Genetic Analysis in Arabidopsis thaliana

该项目将产生一套新颖的、增强的工具（交通线），这将提高对模式植物拟南芥进行基因分析的速度并降低成本。作为一种社区资源，这些线路将促进大量研究者研究这一重要模式生物的广泛生物学问题，并且对高中或大学水平的遗传学基本概念教学也很有用。该项目将为一名博士后和本科生提供培训。交通线（TLs）是拟南芥（Arabidopsis thaliana）的遗传资源，含有表达绿色或红色荧光蛋白的连锁种子特异性转基因。TLs通过使研究人员能够在不需要分子分析的情况下使用干种子识别感兴趣区域的重组体，从而促进遗传作图。这些细胞系使鉴定分离群体中重组染色体纯合的个体成为可能，从而提高了评估这些重组染色体表型的可靠性。这些系也将有助于保持隐性致死等位基因。在哥伦比亚生态型中，通过将紧密相连的pNAP::eGFP和pNAP::dsRED插入到一条染色体上重组，将产生150个以1mb为间隔覆盖整个拟南芥基因组的TLs。十个覆盖基因组更大区域的TLs也将在直立兰伯格中产生。对高中或大学基因重组实验室练习有用的股票也将产生。本项目由分子和细胞生物科学部遗传机制项目资助。

This project will produce a novel, enhanced set of tools (Traffic Lines) that will increase the speed and lower the cost of performing genetic analysis in the model plant, Arabidopsis thaliana. As a community resource, these lines will facilitate the research of a large number of investigators studying a wide range of biological problems in this important model organism, and will also be useful for teaching basic concepts in genetics at a high school or college level. The project will provide training for a postdoctoral fellow and undergraduate students.Traffic Lines (TLs) are genetic stocks of Arabidopsis thaliana that contain linked seed-specific transgenes expressing green or red fluorescent proteins. TLs facilitate genetic mapping by enabling investigators to identify recombinants in a region of interest using dry seeds, without the need for molecular analysis. The lines make it possible to identify individuals homozygous for recombinant chromosomes in a segregating population, increasing the reliability with which the phenotypes of these recombinants can be assessed. The lines also will be useful for maintaining recessive lethal alleles. 150 TLs covering the entire Arabidopsis genome in 1 mB intervals will be generated in the Columbia ecotype by recombining closely-linked pNAP::eGFP and pNAP::dsRED insertions onto a single chromosome. Ten TLs covering larger regions of the genome will also be generated in Landsberg erecta. Stocks useful for a high school or college laboratory exercise in genetic recombination will also be generated.This project is funded by the Genetic Mechanisms Program in the Division of Molecular and Cellular Biosciences.