Spatio-temporal control of light-driven plasmonic and photoswitchable nanoagents (A08)

本项目旨在设计可由光控制操作的纳米试剂。首先，我们将设计一种结合光热操作和超分辨率显微镜的方法来精确测量等离子体纳米结构上的温度分布和控制分子的吸收和解吸。其次，我们将研究如何将等离子体DNA折纸纳米孔纳入脂质双层膜中，用于单分子和DNA的SERS测量。最后，我们将探讨使用光脂作为纳米剂来控制膜的特性。特别是，我们想要研究光开关纳米试剂的双光子光异构化条件，这对于它们在细胞环境和三维矩阵中的应用是必不可少的。

This project aims at the design of nanoagents whose operation can be controlled by light. First, we will devise a method to precisely measure temperature distributions and control molecular absorption and desorption on plasmonic nanostructures by a combination of optothermal manipulation and super-resolution microscopy. Secondly, we will investigate how plasmonic DNA-origami nanopores can be incoorporated into lipid bilayer membranes for SERS measurements of single molecules and DNA. Finally, we will explore the use of photolipids as nanoagents to control characteristic membrane properties. In particular, we want to investigate the conditions for two-photon photoisomerization of photoswitchable nanoagents, which is imperative for their application in cellular environments and 3D matrices.