EAGER: A Novel Method for Simultaneous Enrichment and Detection of Bacterial Cells

PI Ramasamy，Ramaraja P. 提案编号1637863食品加工过程中病原体的快速和早期检测对于防止污染、提高食品安全、质量和卫生具有战略意义。拟议的项目将为这一已确定的问题提供近乎理想的解决方案。在食品加工操作中快速和早期检测病原体对于防止污染、提高食品安全、质量和卫生具有战略意义。拟议的项目将为这一问题制定一个近乎理想的解决方案。一种新的方法，同时分离（富集）和检测的食源性病原体，单核细胞增生李斯特菌，从复杂的食品基质使用噬菌体作为生物识别实体的特点。由于单核细胞增生李斯特菌的可用抗体具有较差的亲合力，PI将为此特定目的对噬菌体进行遗传工程改造。具体的研究目的是：（1）分离L.通过使用重组A511噬菌体从模型食物基质中磁性分离单核细胞增多症。(2)演示快速检测L。A511修饰的电极上的单核细胞增多症使用AC阻抗。 教育和外联目标是扩大妇女对工程的参与，促进STEM学科的多样性，并为工程本科生提供跨学科教育。

PI Ramasamy, Ramaraja P. Proposal No 1637863 Rapid and early detection of pathogens during food processing operations is strategic for preventing contamination, improving food safety, quality and hygiene. The proposed project would provide a near-ideal solution for this identified problem.Rapid and early detection of pathogens in food processing operations is strategic for preventing contamination, improving food safety, quality and hygiene. The proposed project will develop a near-ideal solution to this problem. A novel method for simultaneous isolation (enrichment) and detection of a foodborne pathogen, Listeria monocytogenes, from complex food matrices using bacteriophage as bio-recognition entity will be characterized. Because available antibodies for Listeria monocytogenes have poor avidity, the PI will genetically engineer bacteriophage for this specific purpose. Specific research objectives are: (1) isolate L. monocytogenes by magnetic separation from model food matrix using recombinant A511 phage. (2) Demonstrate rapid detection of L. monocytogenes on A511 modified electrodes using AC impedance. Education and outreach objectives are to broaden participation of women in engineering, promote diversity in STEM disciplines and provide interdisciplinary education for engineering undergraduate students.