NeuroNex Technology Hub: Nemonic: Next generation multiphoton neuroimaging consortium

多光子神经成像是测量大脑神经活动的一种强有力的方法，可以同时提供数千个或更多神经元的亚细胞分辨率。然而，目前的技术存在技术限制，限制了可能进行的实验。因此，该项目将通过名为Nemonic(下一代多光子神经成像联盟)的NeuroNex神经技术中心创造新技术。Nemonic项目包括三个部分。首先，开发部分将在一系列案例研究中创建新系统，以实现目前不可能进行的神经科学实验。其次，传播部分将通过开源资源、研讨会和行业合作伙伴关系将这项技术广泛传播到其他神经科学实验室。第三，先进组件将把多光子神经成像的基础技术推向下一个前沿。将开发两项具体技术：用于多光子神经成像的微型光子系统；以及用于成像亚微米结构的超分辨率成像。此外，一系列会议将促进新的合作，以更快地推进与未来多光子神经成像相关的工程技术。在Nemonic项目中开发的技术也将与制造、3D打印和光子学相关。这项工作还将增加学术界和产业界的合作伙伴关系，加强STEM培训，招募和支持女性和URM科学家的科学培训和职业生涯。案例研究将开发新的仪器，用于大视场双光子和三光子成像、可扩展的时间多路复用和综合行为。这项技术将被开发为与一系列模型系统兼容。重点将放在钙和谷氨酸在细胞体和突起中的成像上，并可以使用其他荧光指示剂。研讨会将涵盖光学设计、制造、组装和使用，面向神经科学家和工程师。一个先进的项目将开发具有变换限制脉冲的高峰值功率超快激光器，具有集成的光束调节、光束控制、聚焦和检测系统。另一个进步项目将开发使用空间频率调制、自适应光学和新的脉冲调节的超分辨率多光子成像。这项工作将通过研究、加强培训和行业合作，共同推动多光子神经成像和一套相关技术的发展。这个神经技术中心奖是由生物科学局新兴前沿司共同资助的，作为大脑倡议和NSF了解大脑活动的一部分。

Multiphoton neuroimaging is a powerful approach for measuring neural activity in the brain, offering subcellular resolution of thousands or more neurons at time. However, current technology has technical limitations that restrict what experiments are possible. Therefore, this project will create new technology through a NeuroNex neurotechnology hub, called Nemonic (NExt generation Multiphoton NeuroImaging Consortium). The Nemonic project has three parts. First, the development component will create new systems in a series of Case Studies to enable currently impossible neuroscience experiments. Second, the dissemination component will spread this technology broadly to other neuroscience labs through open source resources, workshops, and industry partnerships. Third, the advancement component will push the fundamental technology of multiphoton neuroimaging into the next frontier. Two specific technologies will be developed: miniaturized photonic systems for multiphoton neuroimaging; and super-resolution imaging to image submicron structures. Also, a series of meetings will foster novel collaborations to more rapidly advance engineering technologies that are relevant to multiphoton neuroimaging in the future. Technology developed in the Nemonic project will also be relevant to manufacturing, 3D printing, and photonics. This work will also increase partnerships between academia and industry, enhance STEM training, and recruit and support the scientific training and careers of women and URM scientists.The Case Studies will develop new instrumentation for large field-of-view two- photon and three-photon imaging, scalable temporal multiplexing, and integrated behavior. This technology will be developed for compatibility with an array of model systems. The focus will be on calcium and glutamate imaging, in cell bodies and processes, and other fluorescent indicators can be employed. The workshops will cover optical design, fabrication, assembly, and use, for an audience of neuroscientists and engineers. One advancement project will develop high peak power ultrafast lasers with transform limited pulses, with integrated, beam conditioning, beam steering, focusing, and detection systems. The other advancement project will develop super-resolution multiphoton imaging using spatial frequency modulation, adaptive optics, and novel pulse conditioning. Together, this work will advance multiphoton neuroimaging and a suite of related technology, through research, enhanced training, and industry partnerships. This NeuroTechnology Hub award is co-funded by the Division of Emerging Frontiers within the Directorate for Biological Sciences as part of the BRAIN Initiative and NSF's Understanding the Brain activities.