High-speed and minimally photo-damaging STED microscopy using nanolaser arrays

本项目将开发一种快速、经济、小型化的显微镜系统，在最小的光损伤或荧光探针光漂白的情况下，对细胞的精细超微结构进行成像。这一建议的成功将大大推进显微镜领域，特别是超分辨率荧光成像。高性能、快速、紧凑和负担得起的显微镜的发展将影响癌症和阿尔茨海默病等医学诊断。首席研究员将开发下一代受激发射损耗（STED）显微镜，用于高性能，快速，紧凑和负担得起的STED显微镜。PI将金属纳米激光阵列集成到STED系统中，这将大大提高成像速度，减少设备的尺寸和成本。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This project will develop a fast, affordable, and miniaturized microscopy system to image the fine ultrastructure of cells, with minimal photodamage or photobleaching of fluorescent probes. Success of this proposal will significantly advance the field of microscopy, specifically super-resolution fluorescence imaging. The development of high-performance, fast, compact and affordable microscopes will impact medical diagnostics such as cancer and Alzheimer's disease. The principal investigator will develop the next generation of stimulated emission depletion (STED) microscopy for high-performance, fast, compact and affordable STED microscopes. The PI will integrate metallic nanolaser arrays into STED systems which will greatly improve imaging speed and reduce the size and cost of the equipmentThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.