Tailor-made functionalized biomaterials for cell culture applications (bone tissue generation)

该项目旨在开发用于培养骨组织的功能化陶瓷矿物生物基质。功能化应通过用与生物活性配体缀合的水溶性生物相容性但不可降解的聚合物覆盖基质表面来实现，所述生物活性配体例如生长因子如BMP-2和促进细胞粘附的含RGD的肽。特异性配体将通过以下方式影响细胞培养实验：增强细胞附着、改善细胞生长和有助于维持分化。将使用前体模型细胞系（MC 3 T3-E1）在静态细胞培养中测试这些改性复合材料的细胞毒性。因此，将研究细胞增殖和活力。还应测定骨特异性标志物并调查矿化状态。分化状态将通过PCR和DNA微阵列进行研究。从这些筛选实验中获得的最佳候选物将被插入到一个特殊的生物反应器中，用于模型细胞系和成骨细胞的动态和延长培养。

The project aims at the development of functionalized ceramic mineral biomatrices for the cultivation of bone tissue. The functionalization shall be achieved by covering the matrix surface with water soluble biocompatible but non-degradable polymers conjugated to bioactive ligands e.g. growth factors like BMP-2 and cell adhesion promoting RGD-containing peptides. The specific ligands shall influence cell culture experiments by: enhancing cell attachment, improving cell growth and contribution to maintain differentiation. These modified composite materials will be tested in static cell culture using a precursor model cell line (MC3T3-E1) with regard to cytotoxicity. Therefore cell proliferation and viability will be studied. Also bone specific markers shall be determined and mineralization status will be investigated. The differentiation status will be studied by PCR and DNA-microarrays. The best candidates from these screening experiments will be inserted to a special bioreactor for a dynamic and enlongated cultivation of a model cell line and osteoblasts.