Central facility for mass spectrometry and proteomics

中心项目Z4N将通过最先进的质谱技术支持CRU 216的所有项目。我们将应用定量质谱鉴定特定的蛋白质相互作用的合作伙伴（例如，Ral蛋白），为小分子药物（例如，dionconquinones）的细胞靶点的识别，并在翻译调控的背景下分析新合成的蛋白质。我们将建立一个先进的磷酸化蛋白质组学的工作流程，并应用珠偶联广泛选择性激酶抑制剂的激酶的选择性富集。我们将应用磷酸化蛋白质组分析，例如，用于识别激酶NIK和IKK1的直接和间接靶标，以及用于阐明Raf激酶的MEK独立信号转导。

The central project Z4N will support all projects of the CRU 216 with state-of-the-art mass spectrometry. We will apply quantitative mass spectrometry for the identification of specific protein interaction partners (for example, of Ral proteins), for the identification of cellular targets of small molecule drugs (for example, of dioncoquinones), and for the analysis of newly synthesized proteins in the context of translational regulation. We will set up an advanced workflow for phosphoproteomics and for the selective enrichment of kinases applying a bead-coupled broadly selective kinase inhibitor. We will apply phosphoproteome analyses, for example, for the identification of direct and indirect targets of the kinases NIK and IKK1, and for elucidating MEK-independent signal transduction of Raf kinases.