PFI-TT: Accelerated Detection of Successful Crop Production

创新伙伴关系-技术转化（PFI-TT）项目的更广泛影响/商业潜力是通过消除一个关键障碍来加速现代作物生产。目前，产生新作物品系所需的时间和金钱投资造成了对最新改良品种的可及性不平等。通过该项目，将开发一种技术，以减少商业生产管道的时间和成本。这项技术将有助于在分子水平上了解作物生产管道的最早步骤，从而可以快速识别注定失败的项目和生产线，并从生产线上移除。这种早期质量控制将减少与作物生产管道相关的时间和投资，减少该过程中的商业投资，并改善潜在新作物的可及性。拟议的项目旨在在转基因过程后的早期事件分类阶段检测新作物系中的off-type或“异常”rna。这些异常RNA是不需要的，因为它们破坏了细胞的RNA质量控制机制，抑制了这些区域未来的转录。该项目的总体科学目标是开发一种技术，可以检测由植物转基因或任何感兴趣的位点产生的所有RNA，然后利用这些预测信息通知育种者哪些品系应该在作物生产管道中进行进展。研究目标是改进技术，以识别由转基因产生的所有种类的RNA，然后在行业合作伙伴提供的实际作物品系上测试这种预种植潜力。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this Partnerships for Innovation - Technology Translation (PFI-TT) project is the acceleration of modern crop production by removing a key roadblock. Currently, the time and monetary investment required to generate a new crop line produces accessibility inequities to the latest improved strains. Through this project, a technology will be developed to reduce the time and cost to the commercial production pipeline. This technology will help understand the earliest steps of the crop production pipeline on the molecular level, so that projects and lines that are destined to fail can be quickly identified and removed from the production line. This early quality control will reduce the time and investment associated with the crop production pipeline, reducing the commercial investment in the process and improving accessibility to potential new crops. The proposed project aims to detect off-type or “aberrant” RNAs in new crop lines in the early event sorting phase, after the process of transgenesis. These aberrant RNAs are unwanted, as they fail the cell’s RNA quality control mechanism and inhibit future transcription from these regions. The overall scientific goals of this project are to develop a technology that detects all the RNA generated by a plant transgene or any locus of interest, and then use this predictive information to inform breeders which lines should progress in the crop production pipeline. The research objectives are to refine the technology to identify all species of RNA generated by a transgene, and then test this predive potential on real-world crop lines provided by an industry partner.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.