RAPID: NMR Characterization of SARS-CoV-2 Proteins

该奖项授予康涅狄格大学，支持有助于了解非结构蛋白在SARS-COV-2病毒中的作用的研究。研究人员将使用先进的化学分析技术来观察溶液中的蛋白质结构，以补充其他正在进行的研究，研究冷冻或结晶蛋白质样本。这些研究的结果将为我们了解SARS-COV-2生物学提供重要信息，并可能有助于为治疗方法的选择和开发提供信息。作为更广泛的影响计划的一部分，这些研究的数据将通过公开可用的存储库迅速传播，以便其他研究人员可以在自己的研究中利用实验结果。这些资源和数据也将用于支持研究生培训活动。研究结果将发表在同行评议的期刊上，并在科学会议上分享。本项目将利用异源蛋白表达系统和核磁共振（NMR）技术研究SARS-COV-2基因组编码的非结构蛋白的作用。利用核磁共振光谱对溶液中的动力学和无序性进行经验评估，以补充或验证基于晶体结构的预测，并深入了解催化机制、变构调节和抗原性。将进行化学位移分析研究，以帮助鉴定与SARS-CoV-2蛋白结合的代谢物，以推进功能注释。本研究产生的资源和数据将存入生物磁共振数据库（BMRB）或通过其他方式作为公共资源共享。该RAPID奖由生物基础设施部（DBI）使用《冠状病毒援助、救济和经济安全（CARES）法案》的资金颁发。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The award to the University of Connecticut supports research that will help in understanding the role of non-structural proteins in the SARS-COV-2 virus. The researchers will use advanced chemical analytic techniques to look at protein structure in solution to complement other on-going research that looks at either frozen or crystallized protein samples. Results from these studies will contribute vital information to our understanding of SARS-COV-2 biology and may help to inform on the selection and development of therapeutic treatments. Data from these studies will be rapidly disseminated through publicly available repositories as part of the broader impact plan so that other researchers can leverage the experimental outcomes in their own studies. The resources and data will also be used to support graduate student training activities. The study findings will be published in peer-reviewed journals and shared at scientific meetings.This project will use heterologous protein expression systems and nuclear magnetic resonance (NMR) techniques to investigate the role of non-structural proteins encoded by the SARS-COV-2 genome. Empirical assessment of dynamics and disorder in solution using NMR spectroscopy will be used to complement or validate predictions based on crystal structures, and yield insight into catalytic mechanism, allosteric regulation, and antigenicity. Chemical shift profiling studies will be performed to aid in the identification of metabolites that bind to SARS-CoV-2 proteins to advance functional annotation. Resources and data generated from this study will be deposited in the Biological Magnetic Resonance Data Bank (BMRB) or shared through other means as a public resource. This RAPID award is made by the Division of Biological Infrastructure (DBI) using funds from the Coronavirus Aid, Relief, and Economic Security (CARES) Act.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.