Characterization of the cell growth activity of tissue cells in 3D scaffolds with integrated sensors

许多生物学问题（药物发现、毒性测试等）不能用二维细胞培养充分回答。因此，在2D培养中获得的测试结果只能部分地转移到3D或体内的情况。即使有许多描述良好的3D模型，对3D文化的在线表征仍然是困难的。因此，该项目的目标是开发一种具有明确几何结构和集成传感器的支架，用于测量阻抗，作为动物和人类细胞3D培养细胞生长活性的指标。这种具有集成传感器的支架将成为药物筛选的宝贵工具，例如用于评估抗癌药物。在这里，考虑到不同的细胞培养模型在三维生长，如肿瘤细胞模型或肝脏模型。预期支架的定义几何结构被设计为允许基本均匀的流动条件，以及确保支架内细胞的供应。通过阻抗测量，将评估细胞的生长和分布。这些测量结果将与二维模型进行比较。

Many biological problems (drug discovery, toxicity testing, etc.) cannot be answered with 2D cell cultures adequately. As a consequence, test results obtained in 2D culture are only par-tially transferable to the situation in 3D or in vivo. Even if there are a number of well-described 3-D models, still an on-line characterization of the 3D culture is difficult. Therefore goal of the project is to develop a scaffold with a defined geometric structure and integrated sensors for measuring the impedance as indicator for cell growth activity of the 3D culture of animal and human cells. Such a scaffold with integrated sensors would be a valuable tool for drug screening, e.g. for evaluation of anticancer drugs. Here, different cell culture models growing in 3D come into consideration, such as tumor cell models or liver models. The defined geometric structure of the intended scaffold is designed to allow for substantially uniform flow conditions, as well as to ensure a supply of the cells within the scaffolds. By impedance measurements, growth and distribution of the cells will be evaluated. The results of these measurements will be compared with 2D models.