Designing collagen-inspired template-[protein]3-hybrids: From switchable high molecular weight nanostructures towards processing collageneous materials for cell culture.

该联合项目的目标是合成具有高分子量的胶原蛋白启发的模板蛋白杂交体。可切换的模板可以激活自组装成具有胶原三螺旋的各向异性纳米物体。将这些物体加工成纤维、非织造布或薄膜，将提供具有定向内部结构的宏观纳米结构材料。重组蛋白生产的胶原单链将与有机合成模板相结合，以预先定义结构的形成。可切换模板的使用应该允许调节三螺旋的形成，从而激活各向异性纳米物体。作为该项目的一部分，胶原三螺旋的模板导向自组装将集成到湿法、静电纺丝或铸造成型等加工方法中，将纳米级自组装与宏观加工联系起来。从而构建具有向列细结构的宏观纳米结构材料。表征这些材料的分层结构水平和各向异性力学性能是很重要的。大量统一定义的模板-蛋白质杂交体的生产和加工不仅有助于对受控软物质结构形成过程和纳米结构材料加工的基本理解，而且还将使生物材料成为可能。后者将在初步研究中进行调查，阐明材料-细胞相互作用。

Objectives of the joint project are the synthesis of collagen-inspired, template-protein hybrids with high molecular weight. Switchable templates enable activation of self-assembly into anisotropic nano-objects with collageneous triple helices. The processing of these objects to fibers, nonwovens or films will provide macroscopic nanostructured materials with directional internal structure.Recombinant protein production of collageneous single strands will be combined with organic synthesis of templates to predefine structure formation. The use of switchable templates should allow regulation of triple helix formation and thus enables activation of anisotropic nanoobjects.As part of the project, the template-directed self-assembly of collageneous tripelhelices will be integrated into processing methods such as wet-, electrospinning or cast molding, connecting the nanoscale self-assembly with macroscopic processing. Thereby, macroscopic-nanostructured materials with nematic fine structure will be constructed. It is important to characterize both the hierarchical structural levels and the anisotropic mechanical properties of those materials.The production and processing of larger quantities of uniformly defined template-protein-hybrids will not only contribute to the fundamental understanding of controlled soft matter structure formation processes and of processing of nanostructured materials, but will also enable access to biomaterials. The latter will be investigated in an initial study, elucidating material-cell interactions.