Understanding the protein composition and dynamics of the nuclear envelope in plants

核膜（NE）分离和组织基因组。它选择性地积累执行重要细胞功能的蛋白质，代表真核细胞的标志。尽管人们越来越认识到NE是一个重要的结构和信号传导平台，但对NE蛋白的组成和功能的了解有限，特别是在植物中，这极大地限制了我们对植物NE功能及其对植物生理学和细胞生物学各个方面的重要影响的理解。该项目旨在收集模式生物拟南芥中NE膜蛋白的清单，作为全面了解植物NE成分和功能的关键一步。此外，本项目将研究通过特殊的蛋白水解系统调节NE蛋白稳态的分子机制。该研究将为植物和NE生物学研究界提供有价值的NE相关蛋白数据库和创新的蛋白质鉴定工具，并深入吸引STEM和URM本科生在遗传学，蛋白质组学，细胞生物学和分子生物学方面的前沿培训。最近的研究发现了一系列新的植物NE成分及其在不同生物过程中的意义，为研究保守的真核NE功能以及植物特有的NE相关机制提供了新的见解。该项目将采用新开发的接近标记技术，结合无标记定量质谱法，在建立植物NE中蛋白质景观的精确和全面视图方面取得快速进展。结合遗传学、蛋白质组学和细胞生物学方法，该项目还将确定新发现的内核膜相关蛋白降解途径的关键调控成分，该途径对维持NE功能至关重要，为高等真核生物的细胞器特异性膜相关蛋白水解系统增加了一个新的分支。这项研究有望大大提高我们对核膜和核功能的基本生物学原理的理解。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The nuclear envelope (NE) isolates and organizes the genome. It selectively accumulates proteins that carry out vital cellular functions and represents the hallmark of eukaryotic cells. Despite the increased appreciation of the NE as a crucial structural and signaling platform, knowledge on the composition and function of NE proteins is limited, particularly in plants, which significantly limits our understanding of plant NE function and its critical influence on various aspects of plant physiology and cell biology. This project aims to assemble the inventory of NE membrane proteins in the model organism Arabidopsis as a critical step towards a comprehensive understanding of the plant NE constituents and functions. In addition, this project will investigate molecular mechanisms that regulate the NE protein homeostasis through specialized proteolytic system. This research will generate valuable NE-associated protein databases and innovative protein identification tools for the plant and NE biology research community and deeply engage STEM and URM undergraduates in cutting-edge training in genetics, proteomics, cell biology, and molecular biology.Recent studies discovered a range of novel plant NE components and their significance in diverse biological processes, providing new insights into conserved eukaryotic NE functions as well as NE-associated mechanisms unique to plants. This project will deploy the newly developed proximity labeling technology coupled with label-free quantitative mass spectrometry to make rapid advances in establishing a precise and comprehensive view of the protein landscape in the plant NE. Using a combined genetic, proteomic, and cell biological approach, this project will also define key regulatory components of a newly identified inner nuclear membrane-associated protein degradation pathway that is critical in maintaining the NE function, adding a new branch to the organelle-specific membrane-associated proteolytic system in higher eukaryotes. This research promises to significantly advance our understanding of fundamental biological principles underlying the nuclear membrane and nuclear function.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.