Thyroid disruption in developing zebrafish (Danio rerio) - consequences on morphology, physiology and behavior

环境污染物可以扰乱鱼类的甲状腺系统，这一事实已经得到了充分的证实。较少人知道的是甲状腺功能紊乱的发育、生理和行为后果。由于甲状腺系统在生长、发育和繁殖等关键生理过程中发挥着重要作用，其环境诱导的干扰可以影响各种与健康和健康相关的参数，如幼虫的存活、变态、行为或生育。由于选择性死亡，这些生物体水平的变化随后可能传播到改变的人口统计学中。拟议项目的目的是调查启动鱼类甲状腺功能紊乱的分子途径如何导致发育和生理障碍，从而导致适应能力的变化。将研究两种作用模式：干扰甲状腺激素合成，以及与甲状腺激素受体结合。斑马鱼将作为实验模型，应用一套分子、生理和行为方法。

The fact that environmental contaminants can disrupt the thyroid system of fish is well established. Less known are the developmental, physiological and behavioral consequences of thyroid disruption. As the thyroid system plays an important role in key physiological processes such as growth, development and reproduction, its environmentally induced disruption can impact a variety of health and fitness-related parameters such as larval survival, metamorphosis, behavior or fertility. These organism-level changes can then propagate into altered population demography due to selective mortality. The aim of the proposed project is to investigate how initiating molecular pathways of thyroid disruption in fish result in developmental and physiological disturbances leading to fitness changes. Two modes of action will be studied: interference with thyroid hormone synthesis, and binding to thyroid hormone receptors. Zebrafish will be used as experimental model, applying a suite of molecular, physiological, and behavioral approaches.