Rational targeting of intracellular protein transport signals by supramolecular ligands (B05)

在前一个资助期，我们开发了Survivin核输出信号（内斯）的超分子结合剂，以干扰其双重（病理）生物活性。现在，我们打算通过多价性和与多功能纳米载体的偶联来进一步提高这种超分子配体的特异性和活性。此外，我们将通过蛋白酶Taspase1的核输入信号（NLS）的超分子靶向来扩展我们的概念。总的来说，我们的目标是证明，选择性的蛋白质转运调节确实可以实现的信号，而不是受体的超分子靶向。

In the preceding funding period, we developed supramolecular binders to the nuclear export signal (NES) of Survivin to interfere with its dual (patho)biological activity. Now, we intend to further increase the specificity and activity of this supramolecular ligands by multivalency and coupling to multifunctional nanocarriers. Moreover, we will expand our concept by supramolecular targeting of the nuclear import signal (NLS) of the protease Taspase1. Collectively, we aim to demonstrate that selective protein transport regulation can indeed be achieved by supramolecular targeting of signals rather than receptors.