MRI: Acquisition of Q-TOF LCMS for Multidisciplinary Research and Education at Christopher Newport University

该奖项由主要研究仪器和化学研究仪器计划支持。克里斯托弗纽波特大学的罗纳德昆兰教授和同事丽莎韦伯，托德格鲁伯和克里斯托弗Meighan正在获得液相色谱-高分辨率质谱仪，四极杆飞行时间（Q-TOF LCMS）。该提案旨在加强各级研究和教育，特别是在农业和食品化学、神经生物学、发育生物学和生物化学等领域。一般来说，质谱（MS）是用于识别和表征复杂样品中嵌入的少量化学物质的关键分析方法之一。在一个典型的实验中，成分被加热并流入质谱仪，在那里它们被电离。离子的质量被非常精确地测量。这种高灵敏度的技术可以研究复杂混合物中的分子结构。带有液相色谱仪的仪器可以在化合物到达质谱仪之前将其分离。各种电离源提供了关于被分析的样品类型的额外灵活性。此次收购加强了大学和地区的研究基础设施。该工具通过利用这一现代分析技术使不同群体的学生参与研究和研究培训，扩大了参与。特别是，该工具将增强代表性不足和第一代大学生的研究经验。该工具将被纳入四个教学实验室，影响到大量个人，包括代表性不足的群体。该仪器为学生提供了使用重要仪器的经验，他们随身携带到他们的职业生涯。该质谱仪的奖项旨在加强各级研究和教育。它特别影响的研究包括细菌-抗生素相互作用的代谢组学分析，以改善抗生素，塑料降解细菌的代谢组学分析，以允许途径工程将废塑料转化为有价值的化合物，分析模型生物中亚致死浓度的纳米颗粒对激素的影响，膜脂质的定量分析，以更好地了解细胞区室，该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Professor Ronald Quinlan from Christopher Newport University and colleagues Lisa Webb, Todd Gruber, and Christopher Meighan are acquiring a liquid chromatography-high resolution mass spectrometer, quadrupole time-of-flight (Q-TOF LCMS). The proposal is aimed at enhancing research and education at all levels, especially in areas such as agriculture and food chemistry, neurobiology, developmental biology, and biochemistry. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex samples. In a typical experiment, the components are heated and flow into a mass spectrometer where they are ionized. The ions' masses are measured very accurately. This highly sensitive technique allows the structure of molecules in complex mixtures to be studied. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. The various ionization sources provide added flexibility with regard to the types of samples that are analyzed. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse groups of students in research and research training using this modern analytical technique. Particularly, the instrument would enhance research experiences for under-represented and first-generation college students. The instrument would be incorporated into four teaching laboratories impacting a significant number of individuals including those from under-represented groups. The instrument gives students experience using vital instrumentation that they carry with them into their careers.The award of this mass spectrometer is aimed at enhancing research and education at all levels. It especially impacts studies that include metabolomic analysis of bacteria-antibiotic interactions to improve antibiotics, metabolomic analysis of plastic-degrading bacteria to allow pathway engineering to convert waste plastic to valuable compounds, analysis of the effect on hormones of sub-lethal concentrations of nanoparticles in model organisms, quantitative profiling of membrane lipids to better understand cellular compartments, and many additional applications.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.