Preparation and Distribution of DOC Consensus Reference Materials 2022-2025

2022 - 2025年DOC共识标准物质的制备和分发世界海洋中的溶解有机碳（DOC）是地球上活跃循环碳的主要库之一。DOC的碳含量相当于所有大陆生物量的总和。了解这个碳库的运动和变化对了解地球的碳循环至关重要。 测量和跟踪这一大型碳池及其动态的能力需要全世界数十个海洋实验室之间的密切合作。要确保所有这些实验室都能进行准确的DOC测量，就需要有一种方法来确认它们的分析与其他实验室的分析是一致和可比的。该奖项为一项计划提供支持，该计划旨在制定和分发由国际共识设定的DOC浓度海水标准。迈阿密大学将继续实施1998年首次开发的共识参考材料（CRM）计划，以支持国际海洋化学界对溶解有机碳进行一致、高质量的测量。随着这项工作的继续，美国和国际社会将能够继续对一个主要的海洋碳库进行可靠的测量，从而提高社会评估海洋碳循环变化和海洋在气候变化中的作用的能力。该项目为首席研究员实验室的学生和技术人员提供培训机会，并支持他的教学和服务工作。随着国际社会对海洋碳循环研究的持续努力，现有的CRM项目为全球可比的海洋DOC测量提供了必要的基准。将收集来自佛罗里达海峡深处的水（约42 mol/kg DOC），放入安瓿中，并分发给美国和国际社会。自项目启动以来，374，000瓶参考沃茨已分发给45个国家的300个美国和国际实验室，CY21年的分发速度约为27，000瓶/年。 随着该计划的更新，为期3年的支持，来自海洋上层（SSR），中层（MSR）和深层（DSR）沃茨以及低碳参考沃茨（LCW）的参考材料的分配，支持DOC社区的分析工作。此外，在先前的奖项期间开发的新产品将继续：DOC-TN测定试剂盒。该试剂盒配备了分析样品以确定DOC和总氮（TN）所需的所有功能，包括4种碳和氮校准标准品以及4种参考物质（DSR，MSR，SSR和LCW）。它还包括Excel模板，用于指导每次分析运行的标准品、标准物质和样品的顺序。 该工具包满足了那些寻求建立分析能力的人的需求，遵循PI实验室提供的指导方针。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估来支持。

Preparation and Distribution of DOC Consensus Reference Materials 2022-2025Dissolved organic carbon (DOC) in the world ocean is one of the major pools of actively cycled carbon on Earth. DOC holds as much carbon as the living biomass on all continents combined. It is vital to understand the movement and transformations of this carbon pool to understand the Earth’s carbon cycle. The ability to measure and track this large pool of carbon and its dynamics requires close collaboration between dozens of marine laboratories worldwide. Ensuring that all these laboratories are making accurate DOC measurements requires a way to confirm that their analyses are consistent and comparable to the other laboratories. This award provides support for a program that prepares and distributes a seawater standard for DOC concentration that is set by international consensus. The University of Miami will continue a consensus reference material (CRM) program that was first developed in 1998 in support of consistent, high-quality measurements of dissolved organic carbon by the international ocean chemistry community. With continuation of the work, the US and international communities will be able to continue to make reliable measurements of a major marine carbon reservoir, thereby advancing society’s ability to assess changes in the marine carbon cycle and the role of the ocean in climate change. This program provides training opportunities for students and technicians in the lead investigator’s lab and supports his teaching and service work.With continued strong international efforts to investigate the marine carbon cycle, the existing CRM program provides the necessary benchmark for globally comparable marine DOC measurements. Water from the deep Florida Strait (~42 mol/kg DOC) will be collected, placed into ampoules, and distributed to the US and international community. Since program launch, 374,000 vials of reference waters have been distributed to 300 US and international laboratories in 45 nations, at a rate of ~27,000 vials/year in CY21. With renewal of the program for a 3-year period of support, distribution of reference materials from the upper (SSR), mid (MSR) and deep (DSR) waters of the ocean, as well as low carbon reference waters (LCW), supports the analytical work of the DOC community. Further, a new product developed during the prior award will be continued: DOC-TN Determination Kits. The kit comes with all that is necessary to analyze samples for the determination of DOC and total nitrogen (TN), including 4 calibration standards for Carbon and Nitrogen, and 4 reference materials (DSR, MSR, SSR and LCW). It also includes the Excel template, guiding the sequence of standards, CRMs and samples for each analytical run. This kit meets the needs of those who are seeking to build their analytical capabilities, following guidelines provided by the PI’s lab.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.