SBIR Phase II: Bioelectrical Cell Enrichment, Sorting, and Recovery with On-Chip Sample Prep and Monitoring

这个小企业创新研究（SBIR）二期项目的更广泛的影响/商业潜力是一个无标签的生物样品制备和细胞分离平台，用于从各种样品输入中回收稀有细胞。具体来说，通过在单个芯片平台上进行选择性细胞回收的样品制备、操作和监测，将大大减少样品处理步骤，从而确保维持细胞活力，提高样品一致性，并维持天然细胞行为。这种回收的样本对于再生医学和癌症管理中基于细胞的疗法的发展至关重要。个性化医疗还需要精确的细胞恢复。这个小型企业技术转移（STTR）第一阶段项目将开发一个电功能微流控样品操作平台，用于基于细胞的生物物理属性进行表型选择性恢复。具体来说，微流体设计将集成到从复杂的生物输入交换细胞到优化的缓冲中，以实现细胞操作。此外，还将开发用于在表型选择性细胞恢复过程的各个阶段对样品进行芯片监测的仪器。此外，将在设计的设备上验证各种样品输入，以确保转化为商业产品线的性能，一致性和可靠性。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase II project is a label-free, biological sample preparation and cell isolation platform for recovery of rare cells from a variety of sample inputs. Specifically, by carrying out sample preparation, manipulation, and monitoring on a single chip platform for selective cell recovery, the sample handling steps will be greatly reduced, thereby ensuring the maintenance of cell viability, improving sample consistency, and sustaining native cell behaviors. This recovered sample is essential for the development of cell-based therapies in regenerative medicine and cancer management. Personalized medicine also requires precision cell recoveries.This Small Business Technology Transfer (STTR) Phase I project will develop an electrically functional microfluidic sample manipulation platform for phenotype-selective recovery of cells, based on their biophysical attributes. Specifically, microfluidic designs will be integrated to swap cells from complex biological inputs into an optimized buffer to enable cell manipulation. Also, instrumentation will be developed for on-chip monitoring of the sample during various stages of the phenotype-selective cell recovery process. Further, a variety of sample inputs will be validated on the designed devices to ensure performance, consistency, and reliability for translation into commercial product lines.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.