Sustaining the Chlamydomonas Resource Center

明尼苏达大学双城分校的这一奖项支持衣原体资源中心（CRC）的运作，该中心负责维护和分发支持单细胞绿色衣原体研究的资源。 衣原体是一种独特的真核生物模型系统，用于研究光合作用，鞭毛运动和许多其他基本过程，并且是一种越来越多地用于应用研究生物能源和生物燃料的藻类系统。 衣原体也被证明是一个强大的教学工具，该中心鼓励提供一个简单的课堂实验图书馆，并为中学和中学后学校的实验提供廉价的细胞和用品包。 40多年来，在NSF的支持下，CRC为研究人员提供了使用这个强大的模型系统进行研究和教学所需的专业知识和资源。 衣原体资源中心（CRC）是唯一的全面来源的材料和信息需要使用真核绿色衣原体在生物学研究。 这一为期三年的奖项将使CRC能够：1）维护和分发衣原体突变体，细菌克隆和试剂的集合，形成集合的核心; 2）获取，编目，维护和分发新的突变体和野生型菌株和衣原体社区提供的细菌质粒;（三）为教育工作者和学生提供教材和实验方案。由于衣原体保留了动植物细胞的关键祖先特征，包括与高等植物相似的光合作用装置，以及后生动物的纤毛运动特征，衣原体研究的知识影响是非常广泛的。 该中心保存并提供70 000多株衣原体突变株和野生型菌株，沿着cDNA和基因组DNA文库、基因图谱试剂盒、细胞生长试剂以及用于中学和中学后课堂教学的试剂盒和实验室方案。 CRC将所有突变体和野生型衣原体菌株保存在琼脂平板或斜面上，并将两份突变体样本保存在液氮中冷冻。 大多数订单在两天内完成。 该中心还维持一个综合网站（chlamycollection.org），提供一个店面，从该中心的收藏品订购物品，但也提供最新的信息，常用的技术衣原体研究，基因组的遗传和分子图谱，教学工具和新闻感兴趣的衣原体研究界。CRC工作人员通过鼓励用户在出版时将其突变体添加到收集中，并通过接受来自个别研究人员的菌株和菌株收集，确保衣原体研究人员产生的突变体的可用性。 全世界的衣原体研究界都依赖于这一独特的资源来继续和扩大对这一有价值的模型系统的研究。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award to the University of Minnesota Twin Cities supports the operation of the Chlamydomonas Resource Center (CRC), which maintains and distributes resources supporting research on the unicellular green alga Chlamydomonas reinhardtii. Chlamydomonas is a unique eukaryotic model system for studying photosynthesis, flagellar motility, and many other basic processes, and is an increasingly employed algal system for applied research bioenergy and biofuels. Chlamydomonas has also proven to be a powerful teaching tool, which the Center encourages by providing a library of simple classroom experiments, and inexpensive kits with cells and supplies for experiments in secondary and post-secondary schools. For more than 40 years, with NSF support, the CRC has provided the expertise and resources needed by researchers to use this powerful model system for research and teaching. The Chlamydomonas Resource Center (CRC) is the only comprehensive source for materials and information needed to use the eukaryotic green alga Chlamydomonas in biological research. Support from this three year award will allow the CRC to: 1) maintain and distribute the collection of Chlamydomonas mutants, bacterial clones, and reagents that form the core of the collection; 2) acquire, catalogue, maintain and distribute new mutant and wild-type strains and bacterial plasmids provided by the Chlamydomonas community; and 3) to provide teaching kits and experimental protocols to educators and students.Because Chlamydomonas retains key ancestral features of animal and plant cells, including a photosynthetic apparatus much like that of higher plants, as well as ciliary motility characteristic of metazoans, the intellectual impact of Chlamydomonas research is remarkably broad. The CRC maintains and supplies more than 70,000 mutant and wild-type strains of Chlamydomonas, along with cDNA and genomic DNA libraries, kits for genetic mapping, reagents for cell growth and kits and lab protocols for teaching in secondary and post-secondary classrooms. The CRC maintains all mutant and wild-type Chlamydomonas strains on both agar plates or slants and maintains two copies of the mutant collection frozen in liquid nitrogen. Most orders are filled within two days. The CRC also maintains a comprehensive website (chlamycollection.org) that provides a storefront to order items from the CRC collections, but also provides up-to-date information on commonly used techniques for Chlamydomonas research, genetic and molecular maps of the genome, tools for teaching and news of interest to the Chlamydomonas research community. The CRC staff ensures the availability of mutants generated by Chlamydomonas researchers by encouraging users to add their mutants to the collection upon publication, and by accepting strains and strain collections from individual researchers. The Chlamydomonas research community world-wide depends on this unique resource to continue and expand research on this valuable model system.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.