EAGER: PUF-based Probe Design for Secure Access to DNA Storage

一个运转良好的经济取决于所提供的商品和服务的可靠性。从处方药或非处方药到电脑、汽车和电器，所有消费品都有唯一的个人或批号。每个行业使用的原材料都有类似的标识。随着生物经济的发展，有必要对所有使用的生物进行独特识别。这对于建立严格的生物安全和生物安保协议非常重要。该项目将研究一种策略，以唯一地标记单个细胞株，这些细胞株将被遗传，但不能被第三方复制或读取。这将有助于确保一个强健的生物经济的安全。该项目将研究使用DNA作为可寻址的安全数据存储系统的核心。为此，项目团队将追求两个目标。目标1涉及使用crispr技术对细胞系进行基因组编辑，以实现分布式信息存储。他们提议研究细胞系库中物理不可克隆功能（puf）的特性，表征不同的基因组位置，并评估CRISPR编辑的脱靶效应。第二个目标是开发一个片上PUF探针的原型。该探测将有助于确定是否可以有效地实现PUF密钥阵列。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

A functioning economy depends on the reliability of the goods and services being offered. Consumer goods from prescription or over-the-counter drugs to computers, automobiles, and appliances, all have unique individual or batch numbers. Raw materials used in every industry have similar identification labeling. As the bioeconomy develops, it will be necessary to uniquely identify all organisms in use. This will be important to establish strict biosafety and biosecurity protocols. This project will investigate a strategy to uniquely label individual strains of cells that will be inherited but not able to be replicated or read by a third party. This could help ensure the safety and security of a robust bioeconomy.The project will investigate the use of DNA as the core of an addressable, secure data storage system. Towards that end, the project team will pursue two aims. Aim 1 involves the use of CRISPR-technology to perform genome editing of cell lines for distributed information storage. They propose to investigate the properties of physically unclonable functions (PUFs) in a library of cell lines and characterize different genomic locations and assess the off-target effects of CRISPR editing. The second aim is to develop a prototype on-chip PUF probe. This probe will help determine whether a PUF key array can be implemented effectively.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.