Molecular aspects of proton/electron and metabolite supply for chloroplast hydrogenase activity in green algae

植物和蓝细菌利用光合作用将太阳能转化为化学能。在自然界中，化学能储存在各种生物聚合物中。某些绿色藻类已经进化出利用太阳能和氢化酶（H2 ase）从H2O生产H2的额外能力，这对未来生产新的清洁燃料具有明确的意义。本项目的目的是确定控制绿色藻类C光合产氢过程中光子、质子（H+）和电子（e-）流动的关键分子机制。莱因哈德氏菌该计划由六个模块组成，并建立在一个高H2生产突变体（约2%的光子转换效率）。1.大型强子对撞机天线工程将用于建立天线大小和光合效率之间的相关性。2.将通过构建糖转运蛋白突变体来研究饲用氢化酶与外部H+和e-的相关性。3.替代氧化酶将过表达，以评估细胞O2浓度对氢化酶活性的作用。4.新的基因参与氢+和电子供应的氢化酶将被确定和分析的宏阵列和微阵列研究。5.系统发育分析将用于鉴定具有HydA型氢化酶的海藻。6.将评价生理参数对氢化酶活性的影响。

Plants and cyanobacteria use photosynthesis to convert solar energy into chemical energy. In nature, the chemical energy is stored in a diverse range of biopolymers. Certain green algae have evolved the additional ability to use solar energy and hydrogenase (H2ase) enzymes to produce H2 from H2O, with clear implications for production of new clean fuels for the future. The aim of this project is to define key molecular mechanisms controlling photon, proton (H+) and electron (e-) flow during photosynthetic H2 production in the green algae C. reinhardtii. The programme consists of six modules and builds on a high H2 producing mutant (~2% photon conversion efficiency). 1. LHC antenna engineering will be used to establish the correlation between antenna size and photosynthetic efficiency. 2. The relevance of feeding hydrogenases with external H+ and e- will be investigated by construction of sugar transporter mutants. 3. Alternative oxidase will be overexpressed to evaluate the role of cellular O2 concentrations for hydrogenase activity. 4. New genes involved in H+ and e- supply to the hydrogenases will be identified and analysed by macroarray and microarray studies. 5. Phylogenetic analysis will be applied to identify marine algae with HydA-type hydrogenases. 6. Physiological parameters will be evaluated concerning their influence on hydrogenase activity.