Diffusion trapping of neurotransmitter receptors at GABAergic synapses - cell biology and molecular mechanisms (P10)

神经元间突触是大脑中的关键信号单位，而神经递质受体聚集在突触后膜的过程是突触疗效的关键决定因素。我们计划(I)破译细胞黏附蛋白NeuroLogin-2、鸟嘌呤核苷酸交换因子CollyBistin和支架蛋白GePhyrin创建扩散陷阱以在发育中的突触招募和锚定GABAA受体的细胞机制，以及(Ii)确定哪些蛋白质在GABAA能突触上工作，而不需要神经连接素-2或CollyBistin。我们希望我们的研究将导致对GABA能突触的起源及其在健康和疾病中的功能的全面的分子和细胞生物学理解。

Interneuronal synapses are the key signalling units in the brain, and the process of neurotransmitter receptor clustering at postsynaptic membranes is a key determinant of synapse efficacy. We plan (i) to decipher the cellular mechanisms by which the cell adhesion protein Neurologin-2, the guanine nucleotide exchange factor Collybistin, and the scaffold protein Gephyrin create a diffusion trap to recruit and anchor GABAA receptors at developing synapses, and (ii) to determine what proteins operate at GABAergic synapses that do not require Neuroligin-2 or Collybistin. We expect that our studies will lead to a comprehensive molecular and cell biological understanding of the genesis of GABAergic synapses and of their function in health and disease.