The nature and roles of cellular metabolism and energy in the regulation of neuronal chain migration

本研究有两个目标：(1)可视化和量化链迁移神经元的能量状态；(2)揭示促进神经元迁移的代谢来源。对于目标(1)，虽然我已经建立了一个在体内和体外表达遗传编码ATP传感器（ATeam）的系统，但基于fret的细胞内ATP成像的进展受到技术困难的阻碍。为了实现目的(2)，我建立了一个小鼠敲除系，该系缺乏已知在发育过程中促进OXPHOS代谢途径的转录因子。突变系的表型分析显示神经元迁移和/或迁移终止的缺陷。

This research has two objectives: (1) visualize and quantify the energy status of chain migrating neurons; (2) unravel the metabolic sources that fuel neuronal migration.For objective (1), while I have established a system to express genetically encoded ATP sensors (ATeam) in vivo and in vitro, progress in FRET-based imaging of intracellular ATP is hindered by technical difficulties. For objective (2), I have generated a mouse knockout line with deficiency in a transcription factor known to promote OXPHOS metabolic pathway during development. Phenotypic analysis of the mutant line has shown defects in neuronal migration and/or their termination of migration.