Regulatory functions of chloroplast-associated non-coding RNAs in acclimation processes (C03)

我们打算揭示A.thaliana中高光和冷响应的小非编码RNA(SRNA)谱系，并研究它们在调控编码叶绿体定位蛋白的基因中的作用。我们将通过RNA-seq来量化sRNA及其同源靶标的水平，以响应在CRC/TransRegio集中研究的强光和冷处理。我们还将通过RNA-seq来量化SRNA和靶RNA水平，这些突变体具有无序的逆行信号。通过对已发现的与叶绿体相关的sRNA调节回路施加干扰，我们将研究它们在植物适应强光和低温中的作用。

We intend to uncover the high light and cold responsive small non-coding RNA (sRNA) repertoire in A. thaliana and to study their role in the regulation of genes encoding plastid-localised proteins. We will quantify levels of sRNAs and their cognate targets by RNA-seq in response to the high light and cold treatments that are centrally studied in the CRC/Transregio. We will also quantify sRNA and target RNA levels by RNA-seq in selected A. thaliana mutants with disordered retrograde signalling. By imposing perturbations to the identified plastid-related sRNA-regulated circuits we will examine their role in plant acclimation to high light and cold.