Changes in cellular dynamics of cellulose synthases during secondary cell wall production

我们已经提交了一个相关研究项目的草稿。该项目研究了一种与纤维素合成密切相关的蛋白质的作用，但其功能仍然未知，特别是在植物次生细胞壁中。我们的论文提供的数据支持这一假设，即这种蛋白质对控制酶产生纤维素的速度很重要。当蛋白质缺失时，这可能会导致纤维素结晶度的缺陷，导致纤维素强度的下降而不是数量的下降。我们目前正在等待审稿人的回复。我们创造了几种光转换荧光基团标记的纤维素合成酶，并测试了它们在植物中的功效。到目前为止，我们已经确定，尽管这些荧光团中的一些在细菌，酵母和哺乳动物系统中是可行的，但它们在植物中不可行，因为它们的信号弱，并且可能过早地二聚化导致伪影。然而，我们已经确定了几个将有助于我们可视化纤维素合酶的细胞动态周转的目标，并且目前正在利用这些工具开发研究问题，包括确定高尔基体内的酶驻留。我们的实验显示，在发育中的次级细胞壁周围的质膜内存在脂质微域。这些数据推断没有这样的领域，我们目前正在考虑是否可以将这些数据合并在一起发表在一篇短文中。

We have submitted a draft manuscript on one of our related research projects. This project has looked at the role of a protein closely associated with cellulose synthesis, but who’s function is still unknown, especially in plant secondary cell walls. Our paper presents data supporting the hypothesis that this protein is important for controlling the speeds at which enzymes produce cellulose. This may cause the defects in the crystallinity of the cellulose being produced when the protein is absent, resulting in the decrease in cellulose strength but not quantity. We are currently awaiting response from reviewers.We have created several photo-convertible fluorophore tagged cellulose synthase enzymes and tested their efficacy in plants. So far we have determined that though some of these fluorophores are viable in bacteria, yeast and mammalian systems, they are not viable in plants due to their weak signal and potential for dimerizing prematurely causing artifacts. We have however identified several that would work for our goals of visualizing the cellular dynamic turnover of cellulose synthase enzymes and are currently developing research questions with such tools including determining enzyme residency within Golgi bodies.Our experiments have showed negative data for the presence of lipid microdomains within the plasma membrane surrounding developing secondary cell walls. This data infers that there are no such domains and we are currently seeing if we can combine the data to publish in a short paper.