EVOCATE Function and evolution of plant cell wall architecture for sustainable technologies

EVOCATE 可持续技术植物细胞壁结构的功能和演变

• 批准号: EP/X027120/1
• 负责人: Paul Dupree
• 金额: $ 274.41万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FX027120%2F1
• 关键词: EVOCATE; Function; evolution; plant; cell

When land plants evolved from green algae, their cell walls changed. The walls allow plant cells to expand into many shapes for multicellular growth and development, yet these walls also can provide the strength to support tall trees. To achieve the new and varied functions, land plants acquired additional cell wall polysaccharide components. These polymers interact to build distinct molecular arrangements or 'cell wall architectures', providing remarkably different material properties.Function and evolution of plant cell wall architecture for sustainable technologies (EVOCATE) aims to challenge long-held ideas of plant cell wall structure. By studying a range of land plants across evolution, we will investigate the hypothesis that cellulose and other wall polysaccharides have evolved varied and complementary structures that regulate the interactions of these polysaccharides in plant cell walls. EVOCATE will develop and apply advanced solid-state NMR techniques that allow us to measure polysaccharide structures, conformations and polymer interactions in intact cell walls. Our recent studies indicate previously unrecognised important cell wall functions for the polysaccharide known as glucomannan. We have evidence that this polysaccharide is essential for life of all land plants. We propose that the glucomannans interact with distinct types of cellulose fibrils. We will develop and apply genetic methods to manipulate the glucomannan structures to determine the polysaccharide structure-function relationships in assembly of functional cell wall architectures. EVOCATE will thereby develop new principles and models of plant cell wall assembly. We will demonstrate how multiple and varied architectures of cell walls contribute to the robust yet flexible properties that allow land plants to grow. The techniques and discoveries of EVOCATE will therefore underpin development of new applications of biomass for sustainable technologies.This is an ERC advanced Grant Award.

当陆地植物从绿色藻类进化而来时，它们的细胞壁发生了变化。这些细胞壁允许植物细胞扩展成多种形状，以促进多细胞生长和发育，但这些细胞壁也可以提供支撑高大树木的力量。为了实现新的和多样化的功能，陆地植物获得额外的细胞壁多糖成分。这些聚合物相互作用以构建不同的分子排列或“细胞壁结构”，提供显著不同的材料特性。植物细胞壁结构的功能和进化可持续技术（EVOCATE）旨在挑战长期以来对植物细胞壁结构的看法。通过研究一系列陆地植物的进化过程，我们将研究纤维素和其他壁多糖已经进化出多种互补结构的假设，这些结构调节这些多糖在植物细胞壁中的相互作用。EVOCATE将开发和应用先进的固态NMR技术，使我们能够测量完整细胞壁中的多糖结构，构象和聚合物相互作用。我们最近的研究表明，以前未认识到的重要细胞壁功能的多糖称为葡甘露聚糖。我们有证据表明这种多糖对所有陆地植物的生命都是必不可少的。我们认为葡甘露聚糖与不同类型的纤维素原纤维相互作用。我们将开发和应用遗传学方法来操纵葡甘露聚糖结构，以确定功能细胞壁结构组装中的多糖结构-功能关系。因此，EVOCATE将开发植物细胞壁组装的新原理和模型。我们将展示细胞壁的多种多样的结构如何有助于陆地植物生长的强大而灵活的特性。因此，EVOCATE的技术和发现将支持生物质可持续技术的新应用的开发。这是一个ERC高级赠款奖。

项目成果

XAPT and XLPT enzymes modify the glucuronic acid side chains of tissue-specific xylans in Arabidopsis and Eucalyptus

XAPT 和 XLPT 酶修饰拟南芥和桉树中组织特异性木聚糖的葡萄糖醛酸侧链

• DOI: 10.1101/2024.01.23.575660
• 发表时间: 2024
• 作者: Yu L

The biosynthesis, degradation, and function of cell wall ß-xylosylated xyloglucan mirrors that of arabinoxyloglucan

细胞壁α-木糖基化木葡聚糖的生物合成、降解和功能与阿拉伯木葡聚糖相似

• DOI: 10.1111/nph.19305
• 发表时间: 2023
• 期刊: New Phytologist
• 影响因子: 9.4
• 作者: Wilson L

Differing structures of galactoglucomannan in eudicots and non-eudicot angiosperms

真双子叶植物和非真双子叶被子植物中半乳葡甘露聚糖的不同结构

• DOI: 10.1101/2023.06.18.545452
• 发表时间: 2023
• 作者: Ishida K