iPS神経変性疾患モデル－ロボット結合系を利用した経路探索機能再建の試み

细胞组合对多种脑功能至关重要，但这些组合中节点的损伤可能导致功能衰竭。据推测，激活剩余的细胞组件对于恢复失去的功能是必不可少的。激活绕过受损路径的通路可能是实现这一目标的潜在方法。电刺激是激活旁路通路的一种潜在方法，因为它有可能改变受刺激神经元之间的突触连接。该研究旨在建立一个体外平台，研究电刺激如何通过改变神经元组件之间的连接来编码功能。获得了两个主要结果：建立体外神经元系统来研究信息转换和评估系统获取电刺激程序所给予的信息。该系统由新皮层和海马体网络组成，通过突发活动显示出交叉频率耦合特性，这表明该系统可用于研究多组分神经元系统之间的信息转换，发表于《神经科学前沿》。利用这个系统，一种称为“时间间隔训练”的刺激程序被开发出来，以调节神经元之间的尖峰关系，允许调整两个亚种之间的爆发活动传播时间。然后使用实时成像方法来识别不同神经元在突发事件中的参与，为设计的信息占有系统的机制提供了有价值的见解。

Cell assemblies are essential for multiple brain functions, but damage to nodes within these assemblies can cause functional failure. It is hypothesized that activating remaining cell assemblies is essential to restore lost function. Activating pathways that bypass the damaged routes may be a potential method to achieve this. Electrical stimulation is a potential method to activate the bypassing pathway, given its potential to modify the synaptic connection between stimulated neurons.The research aimed to establish an in vitro platform to study how electrical stimulation can encode functions by modifying connections between neuronal assemblies. Two main results were obtained: establishing an in vitro neuronal system to study information transformation and evaluating the system to acquire information given by electrical stimulation programs.The developed system, composed of neocortical and hippocampal networks, demonstrated cross-frequency coupling properties via burst activities, which indicates the usage of the system in studying information transformation between multi-component neuronal systems, as published in Frontiers in Neuroscience.Using this system, a stimulation program called "time interval training" was developed to regulate the spiking relations between neurons, allowing for the adjustment of the burst activities propagation time between the two sub-cultures. Live imaging methods were then used to identify the participation of different neurons in burst events, providing valuable insight into the mechanism of the designed system for information possession.