Localization of double bond positions in complex lipids by hyphenated mass spectrometric techniques

近年来，主要得益于质谱学技术，磷脂或糖脂等复杂脂类的分析取得了很大进展。不过，双债仓位的确定仍是一大挑战。因此，该项目的目标是开发两种方法来定位复杂脂质中的双键位置。一方面，将在光化学反应器中进行基于PaternóBüchi-与丙酮反应的在线转化。另一方面，介质阻挡放电等离子体的反应性应用于双键氧化。杂原子的引入将指导质谱学的裂解，从而可以推算出双键位置。这两种方法都将在线与高效液相分离相结合，通过分析参考化合物来表征，并应用于复杂的脂类提取物(人乳腺癌细胞培养提取物)。

Analysis of complex lipids like phospho- or glycolipids has made great progress in recent years mainly due to mass spectrometric techniques. However, the determination of the double bond positions is still a challenge. Therefore, the aim of the project is the development of two methods for the localization of the double bond positions in complex lipids. On the one hand, an online conversion based on the Paternò Büchi-reaction with acetone in a photochemical reactor will be performed. On the other hand, the reactivity of a dielectric barrier discharge plasma shall be utilized for double bond oxidation. The introduction of heteroatoms shall direct the mass spectrometric fragmentation and thus, double bond position can be deduced. Both methods will be coupled online to a HPLC separation, characterized by analysis of reference compounds and applied to complex lipid extracts (human breast cancer cell culture extracts).