Molecular characterization of transcription factors controlling indolic and aliphatic glucosinolate biosynthese in Arabidopsis thaliana

硫代葡萄糖苷是一类重要的植物次生代谢产物，在植物的生物胁迫反应中具有保护作用，是人类重要的抗癌物质。由于对硫代葡萄糖苷生物合成途径的转录调控以及硫代葡萄糖苷在植物发育过程中的积累以及对环境刺激的响应知之甚少，我们的目标是鉴定和表征拟南芥中硫代葡萄糖苷生物合成调控的新模式。为此，(I)参与控制脂肪族(HAG1和HAG2)和吲哚硫代葡萄糖苷(HIG1和HIG2)生物合成途径的新的转录因子将被鉴定；HAG1/HAG2和HIG1/HIG2属于R2R3-MYB蛋白，是拟南芥中最大的转录因子家族之一，(Ii)将确定构成脂肪族和吲哚硫代葡萄糖苷生物合成途径的相应靶基因，并揭示它们的共同调节；(Iii)将研究HIG1/2和HAG1/2在植物发育过程中和对生物刺激的响应中对这些生物合成途径调控的影响，以及(Iv)将鉴定参与硫代葡萄糖苷生物合成中间体运输的潜在转位基因。

Glucosinolates belong to an important group of plant secondary metabolites that have protective functions in biotic stress response of plants and that are important anticancerogenic substances for humans. Because the transcriptional control of glucosinolate biosynthetic pathways as well as the accumulation of glucosinolates during plant development and in response to environmental stimuli are only poorly understood, we aim to identify and characterize new patterns of regulation of glucosinolate biosynthesis in Arabidopsis thaliana. To this end, (i) novel transcription factors involved in the control of biosynthetic pathways of aliphatic (HAG1 and HAG2) and indolic glucosinolates (HIG1 and HIG2) will be characterized; HAG1/HAG2 and HIG1/HIG2 with so far unknown functions belong to the R2R3-MYB proteins comprising one of the largest families of transcription factors in A. thaliana, (ii) the corresponding target genes constituting aliphatic and indolic glucosinolate biosynthetic pathways will be identified and their coregulation unraveled, (iii) the impact of HIG1/2 and HAG1/2 on the regulation of these biosynthetic pathways during plant development and in response to biotic stimuli will be studied, and (iv) a putative translocator involved in the transport of glucosinolate biosynthetic intermediates will be characterized.