300 keV FEG Electron Cryo Microscope for Tomography

分子和细胞电子低温断层扫描（cryoET）可以直接在它们的天然环境和它们的相互作用伙伴的天然浓度下研究大分子。这对于基本生物过程的机制分析尤其重要。在现代综合结构生物学方法中，结合了一系列方法来回答生物学问题，cryoET扮演着核心角色，因为它位于（超分辨率）荧光显微镜的动态信息和x射线晶体学、核磁共振和单粒子冷冻电镜的最高分辨率结构信息的十字路口。在感染生物学问题的背景下（汉堡的一个主要研究重点），低温低温技术为研究不同水平的宿主-病原体相互作用提供了一个中心工具。在冷冻et，综合和相关综合冷冻显微镜方面有各自的专业知识，并通过招募Kay gr<s:1> newald在汉堡进一步发展。较厚细胞样品的CryoET具有特定的硬件和软件要求。这些包括非常稳定的样品级与机器人样品变化，高精度倾斜，能量滤波，相板和最新一代的直接电子检测器允许电子计数。与单粒子项目相比，低温低温et项目更耗时，因此，在主要用户和具有各自生物应用的其他大学团体之间结合起来，是一种将充分利用的专用和互补仪器。

Molecular and cellular electron cryo tomography (cryoET) enables studies of macromolecules directly in their native environment and under native concentrations of their interaction partners. This is of particular relevance for mechanistic analyses of basic biological processes. In a modern integrative structural biology approach that combines a range of methods to answer a biological questions, cryoET takes a central role, as it sits at the cross road of dynamic information from (super-resolution-) fluorescence microscopy and highest resolution structural information from X-ray crystallography, NMR und single particle cryoEM. In the context of infection biology questions (a major research focus in Hamburg) cryoET provides a central tool for the investigation of the various levels of host-pathogen interactions. There is respective expertise in cryoET, integrative and correlative integrative cryo microscopies and their further advancement in Hamburg through the recruitment of Kay Grünewald. CryoET of thicker cellular samples has specific hard and software requirements. These include very stable sample stages with robotic sample changes, high-precision tilting, energy filtering, phase plates und latest generation direct electron detectors allowing for electron counting. In comparison to single particle projects cryoET projects are more time consuming and hence justify, combined between a major user and the range of other university groups with respective biological applications, a dedicated and complementary instrument that will be used to full capacity.