Bildgebendes Massenspektrometer

为了阐明生物过程的机制，组织样品的分子表征具有极大的意义。除了定性和定量描述所涉及的分子，这些生物分子的空间分布的知识是必不可少的，我们复杂的细胞组织和动力学的理解。为此目的而出现的一种技术是成像质谱法，作为一种无需分析物先验知识的无标记技术。杜塞尔多夫海因里希-海涅大学（HHU）没有具有成像能力的质谱仪。HHU的以下项目需要使用质谱仪进行高分辨率（5 μm）MALDI成像，并具有MS/MS功能，以详细表征分析物：通过细胞特异性寡糖质量分析（COLIMP）研究植物细胞壁的精细结构以及使用该技术来研究过多的细胞壁突变体和特化细胞，例如参与C4光合作用的细胞。通过肽谱、脂质和甾醇谱对特定C4蛋白复合物进行细胞特异性测定。干细胞信号分子如CLE的动力学和翻译后修饰。

For the elucidation of the mechanism of biological processes the molecular characterization of tissue samples is of great interest. Besides a qualitative and quantitative description of involved molecules the knowledge about spatial distribution of these biomolecules is essential for our understanding of complex cellular organisation and dynamics. One technique that has emerged for this purpose is imaging mass spectrometry as a label-free technique without prior knowledge of the analyte. The Heinrich-Heine University of Düsseldorf (HHU) does not possess a mass spectrometer with imaging capabilities. The use of a mass spectrometer for a high-resolution (5 μm) MALDI-imaging with MS/MS capabilities for the detailed characterization of the analyte is required for the following projects at the HHU: Investigation of the fine structure of the plant cell walls of various cells and parts thereof by cell-specific oligosaccharide mass profiling (COLIMP) and the use of this technique to investigate a plethora of cell wall mutants and specialized cells e.g. those involved in C4 photosynthesis. The cell specific determination of specific C4 protein complexes by their peptide profiles and lipid and sterol profiles. The dynamics and posttranslational modifications of stem cell signalling molecules such as CLE.