Proteins on nanocarriers: from the stealth effect to active targeting in vivo (B11*)

从观察到一些纳米载体在血清蛋白的背景下几乎瞬时地建立蛋白质冠，这对细胞摄取和细胞内运输具有严重的结果开始，该项目针对三个不同的主题。首先，它将研究可能的电晕形成更多的载体系统，以确定这种现象的普遍性。然后，申请人希望寻找在冠中预组织所需蛋白质以改变生物分布的方法。最后，目的是以化学方式结合所需的蛋白质如簇蛋白和/或抗体，以获得-以这种方式-类似隐形的行为。

Starting with the observation that some nanocarriers build up a protein corona nearly instantaneous-ly in context with serum proteins, which has serious results for cellular uptake and intra-cellular trafficking, this project aims at three different topics. At first it will study possible corona formation on more carrier systems to determine the generality of this phenomenon. Then the applicants want to look for ways to pre-organize desired proteins in the corona to modify biodistribution. At last it is the aim to bind desired proteins like clusterin and/or antibodies chemically to obtain –in this way- a stealth like behavior.