The role of small proteins in the plant pathogen Xanthomonas

γ变形杆菌黄单胞菌。胡椒粉和番茄上的白斑病引起细菌性斑疹病。基于RNA-seq分析，我们发现了200个转录本，这些转录本可能含有一个或几个预测的小orf，并可能编码µ蛋白（10-50个氨基酸，aa）。这些转录本中的49个可能受到III型分泌系统的关键调节因子的调节，这对Xcv致病性至关重要。我们的目的是鉴定翻译的sorf和微蛋白在Xcv毒力中可能发挥的作用。具体目标是：(i)全基因组鉴定翻译的sorf和相应的微蛋白（ii）鉴定潜在分泌的微蛋白（iii）生物信息学分析、表达和定位研究以及选定候选微蛋白的功能分析，以鉴定与Xcv毒力有关的微蛋白。（iv）微蛋白相互作用伙伴的鉴定

The gamma proteobacterium Xanthomonas campestris pv. vesicatoria causes bacterial spot disease on pepper and tomato. Based on RNA-seq analyses we identified 200 transcripts that potentially harbor one or several predicted small ORFs and might encode µ-proteins (10-50 amino acids, aa). 49 of these transcripts are presumably regulated by the key regulators of the type III secretion system, which is essential for Xcv pathogenicity.We aim at the identification of translated sORFs and µ-proteins with possible role in Xcv virulence. The specific aims are:(i) Genome-wide identification of translated sORFs and corresponding µ-proteins(ii) Identification of potentially secreted µ-proteins (iii) Bioinformatic analyses, expression and localization studies and functional analysis of selected candidates to identify µ-proteins involved in Xcv virulence.(iv) Identification of µ-protein interaction partners