Nonhost resistance of Arabidopsis thaliana to Phytophthora infestans

拟南芥能够成功地预防马铃薯晚疫病病原疫霉（Phytophthora infestans）的感染和定植。这种非宿主的抵抗是持久的，基于几层防御。入侵前抗性是第一层，涉及到从吲哚硫代葡萄糖苷中产生吲哚化合物的黑芥子酶PEN2的活性。病原菌成功侵入pen2突变体植物，导致防御反应增强。为了确定非寄主抗性所需的其他基因，最好是在入侵后水平，对pen2突变体诱变种子的后代进行筛选，以观察它们对感染P. infestans的反应。分离出14个反应增强的突变体。通过图谱克隆和全基因组测序鉴定受影响的基因，以及随后对编码蛋白的分子分析，将有助于深入了解成功防御病原菌的机制。

Arabidopsis thaliana is able to successfully prevent infection and colonization by Phytophthora infestans, the causal agent of late blight disease of potato. This nonhost resistance is durable and based on several layers of defense. Pre-invasion resistance is the first layer and involves the activity of the myrosinase PEN2 which produces indole compounds from indole glucosinolates. Successful penetration of pen2 mutant plants by P. infestans leads to enhanced defense responses. To identify additional genes required for nonhost resistance, preferably at the post-invasion level, the progeny of mutagenized seeds of the pen2 mutant was screened for alterations in their response to P. infestans infection. Fourteen mutants with enhanced responses were isolated. The identification of the affected genes by map-based cloning and whole genome sequencing as well as the subsequent molecular analysis of the encoded proteins will provide insight into the mechanisms of successful defense against P. infestans.