Identification and molecular characterization of microbe-derived PAMPs and their corresponding perception systems in Arabidopsis

PAMP触发的免疫（PTI）构成了主要或基础的植物免疫应答。PTI的激活基于植物模式识别受体对微生物表面的不变模式的识别。尽管鉴定了一些在各种植物物种中触发免疫反应的微生物表面组分，但预计植物的非自我识别能力将大大超出目前的知识。在以前的工作中，我们已经确定了青枯雷尔氏菌细胞提取物的分级肽片段，触发免疫相关的反应，在拟南芥。这些肽将被纯化至均一并用于鉴定相应的平台模式识别受体。革兰氏阳性和革兰氏阴性细菌衍生的肽聚糖（PGN）诱导拟南芥的PTI反应。LYM3是一种拟南芥质膜蛋白，携带两个异位LysM结构域，与PGN结合、PGN敏感性和植物对细菌感染的免疫性有关。LYM3是包含两个额外成员（LYM1、LYM2）的小蛋白质家族的成员。我们的目标是表征LYM1和LYM2蛋白对PGN感知和植物对微生物感染的免疫的贡献。

PAMP-triggered immunity (PTI) constitutes the primary or basal plant immune response. Activation of PTI is based upon the recognition of invariant patterns of microbial surfaces by plant pattern recognition receptors. Despite the identification of some microbial surface components that trigger immune responses in various plant species, non-self recognition capacities of plants are expected to reach substantially beyond current knowledge. In previous work, we have identified in fractionated Ralstonia solanacearum cell extracts peptidic fragments that trigger immunity-associated responses in Arabidopsis. These peptides will be purified to homogeneity and used to identify the corresponding plattern pattern recognition receptor.Gram-positive and Gram-negative bacteria-derived peptidoglycans (PGN) induce PTI responses in Arabidopsis. LYM3, an Arabidopsis plasma membrane protein that carries two ectopic LysM domains, has been implicated in PGN binding, PGN sensitivity and plant immunity to bacterial infection. LYM3 is a member of a small protein family comprising two additional members (LYM1, LYM2). We aim at characterizing the contribution of LYM1 and LYM2 proteins to PGN perception and to plant immunity to microbial infection.