Preparation and characterisation of bioactive anion-modified hydroxyapatite particles: Protein adsorption mechanism on biomaterials

离子取代的羟基磷灰石颗粒可以作为蛋白质的载体及其控制释放用于骨再生，这是公认的。人们对无机载体纳米颗粒的表面结构（分子群）知之甚少，最重要的是，对蛋白质与这些表面的结合机制知之甚少。该项目旨在通过结合BAM和冈山大学两个研究团队的专业知识，研究(i)硼酸盐取代HAp的结构，特别是表面位点，以及（ii）从这种修饰的HAp颗粒中释放蛋白质的机制。日本站点专注于材料的制备和蛋白质吸附测量，而柏林术语负责所有结构表征，最好使用固态核磁共振，包括首次尝试探索使用未标记样品监测蛋白质吸附/结合的可能性。

It is well accepted that ion-substituted HAp particles can be used as carriers of proteins and their controlled release for bone regeneration. Very little is known of the structure of the surface sites (molecular groups) of the inorganic carrier nanoparticles and, most importantly, of the protein binding mechanism to these surfaces. This project aims to investigate (i) the structure, particularly of the surface sites of borate substituted HAp and (ii) the mechanism of the protein release from the such modified HAp particles by combining the expertise of the two research teams at the BAM and in Okayama. The Japanese site focuses on the preparation of the materials and the protein adsorption measurements whereas the Berlin term is responsible for all structural characterisations, preferably using solid state NMR, including first efforts to explore the possibilities for monitoring the protein adsorption/binding using unlabelled samples.