Patho-physiological study of piloplasmosis in domestic animals

本文对吉氏巴贝斯虫感染犬的非寄生性红细胞破坏和血小板减少的机制进行了研究。研究结果如下：1.研究结果。感染犬红细胞膜蛋白条带4.1a较正常犬明显减少。结果：1.感染犬红细胞膜胆固醇和磷脂浓度均高于正常犬。当吉氏杆菌与红细胞共同培养时，培养物中高铁血红蛋白(MetHb)和丙二醛浓度显著增加，同时伴随着寄生虫的高增殖，但在培养物中寄生虫的低繁殖率时则不明显。感染犬红细胞与乙酰苯肼孵育后，感染后第3天红细胞MetHb生成量明显高于接种后第0天。在犬富含血小板的血浆中加入吉氏杆菌感染的红细胞或其培养上清液，可促进其与ADP的聚集。提示吉氏杆菌感染犬的非寄生性红细胞对氧化应激敏感，这种促进犬血小板聚集的因素可能与犬巴贝斯虫病时出现的血小板减少有关。

The mechanisms of destruction of nonparasitized erythrocytes and thrombo-cytopenia in dogs infected with Babesia gibsoni were studied. The results were as follows.1. The membrane proteins from erythrocytes of the infected dogs showed a markedly reduced level of band 4.1a compared to that from normal dogs. The concentrations of both cholesterol and phospholipids in the erythrocyte menbranes from the infected dogs were higher than those from normal dogs.2. When B.gibsoni was cultured together with erythrocytes, marked increase of methemoglobin (metHb) and malondialdehyde concentrations of erythrocytes in the cultures were observed together with high multiplication of the parasites, but not at low multiplication of the parasites in the culture. When erythrocytes from the infected dogs were incubated with acetylphenyl-hydrazine, the metHb formation in erythrocytes was much higher at day 3 than at day 0 after the inoculation of the parasites.3. Addition of erythrocytes or the supernatant from B.gibsoni-infected erythrocyte culture to canine platelet-rich plasma showed enhanced platelet aggregation with ADP.These results indicate that nonparasitized erythrocytes of dogs infected with B.gibsoni are sensitive to oxidative stress, and that the factor which promoted canine platelet aggregation may be associated with thrombocytopenia observed in canine babesiosis.