気候変動による熱波が珊瑚礁の魚に及ぼす影響の解明

首先，我是一篇论文的第一作者，该论文对海葵鱼克氏Amphiprion clarkii的染色体尺度基因组进行了测序、生物信息学组装和分析。该项目产生了一个高质量的基因组资源，将用于OIST和世界各地其他研究小组的研究。这些结果发表在G3: Genes | genomics | Genetics上。我完成了一个实验，调查海洋变暖对小丑鱼幼虫发育的影响。这包括进行一项以水族馆为基础的研究，将幼虫暴露在不同的温度环境中，并记录它们的反应。我发现幼虫生长发育得更快，代谢率更高，许多基因在更高的温度下表达不同。这项实验的结果和随后的生物信息学分析发表在《整体环境科学》杂志上。此外，我目前正在完成这个实验的第二份手稿，其中这些幼虫的组织特异性分子反应是用rna测序来评估的。本文将于2023年4月提交给《全环境科学》杂志。在幼虫实验之后，我完成了一个研究海洋热浪对小丑鱼幼鱼影响的实验。这个长达4个月的实验已经完成，并进行了代谢测量和rna测序取样。基因组数据现已收到，正在等待生物信息学分析，该分析将于2023年5月完成，然后于2023年7月公布这些结果。

Firstly, I was the lead author on a publication in which the chromosome-scale genome of the anemonefish Amphiprion clarkii was sequenced, bioinformatically assembled and analyzed. The project produced a high-quality genomic resource that will be used for research at OIST and other research groups around the world. These results were published in G3: Genes | Genomes | Genetics.I completed an experiment that investigated the impact of ocean warming on larval development of clownfish. This involved conducting an aquaria-based study in which larvae were exposed to different temperature regimes, and their response recorded. I found that larvae grow and develop much faster, display elevated metabolic rates and have many genes differentially expressed at higher temperatures. The result of this experiment and subsequent bioinformatic analyses were published in the journal Science of the Total Environment. Furthermore, I am currently finalizing the second manuscript from this experiment, in which the tissue-specific molecular response of these larvae was assessed with RNA-sequencing. This paper will be submitted to the journal Science of the Total Environment in April 2023.Following up on the larval experiment I completed an experiment investigating the impact of marine heatwaves on juvenile clownfish. This 4-month long experiment has been completed, with metabolic measurements and sampling for RNA-sequencing conducted. Genomic data has now been received and is awaiting bioinformatic analyses, which will be completed in May 2023, before publication of these results in July 2023.