Development of perfusion preservation method of liver graft, aiming to clinical application for liver transplantation using non-heart-beating-donor

我们证实，在大鼠肝脏和心脏的简单冷保存中，由于抑制了细胞内钙超载和细胞骨架的破裂，并刺激了有氧呼吸，这种新的溶液比UW溶液具有优越性。然而，在大动物模型的肝、肾保存和移植中，由于灌流不足，这些有益作用消失了，这可能与高浓度的聚乙二醇有关。为了继续进行临床应用，应该探索定制聚乙二醇浓度。灌流保存可能是解决这个问题的一种方法。解决了各种技术难题，建立了猪肝脏灌流保存与移植模型。

We confirmed that the superiority of the novel solution over UW solution for the use of simple cold preservation in rat liver and heart, due to the inhibition of cytosolic Ca2+ overload and cytoskeletal breakdown, and stimulation of aerobic respiration. However, these beneficial effects were abolished in liver and kidney preservation and transplantation of the large animal models, due to insufficient perfusion, which is possibly attributed with high concentration of PEG. To proceed for clinical application, customization of PEG concentration should be explored. Perfusion preservation might be a solution for this problem. Thus, the model of liver perfusion preservation and transplantation in pigs has been established with resolving various technical problems.